Tervo T, Palkama A
Acta Anat (Basel). 1978;102(2):164-75.
The innervation of the rabbit cornea was investigated histochemically and electron-microscopically with special reference to the autonomic nerves. Both formaldehyde- and glyoxylic-acid-induced fluorescence methods revealed adrenergic nerves in the stroma; a few fibres were also observed between the basal epithelial cells near the limbus. Acetylcholinesterase- (AChE-) positive nerves were found both in the stroma and in the epithelium, whereas nonspecific cholinesterase (NsChE) activity appeared only in the stromal nerves. Under the electron microscope, both AChE and NsChE activities were observed to be located in the axon membranes. A weak NsChE reaction also appeared in the Schwann cells. When the specimens fixed with KMnO4 were examined under the electron microscope, most nerve fibres did not contain any special axoplasmic structures, although several axons contained mitochondria. Moreover, two vesicle-containing axon types were found in the stromal nerves; axons with small granular vesicles and axons containing small agranular vesicles. In the epithelium, two types of fibres were observed; one type containing only mitochondria while the other showed both agranular vesicles and mitochondria.
利用组织化学和电子显微镜技术，特别针对自主神经，对兔角膜的神经支配进行了研究。甲醛诱导荧光法和乙醛酸诱导荧光法均显示基质中有肾上腺素能神经；在角膜缘附近的基底上皮细胞之间也观察到少量纤维。在基质和上皮中均发现了乙酰胆碱酯酶（AChE）阳性神经，而非特异性胆碱酯酶（NsChE）活性仅出现在基质神经中。在电子显微镜下，观察到AChE和NsChE活性均位于轴突膜上。雪旺细胞中也出现了微弱的NsChE反应。当用高锰酸钾固定的标本在电子显微镜下检查时，大多数神经纤维不含任何特殊的轴浆结构，尽管有几条轴突含有线粒体。此外，在基质神经中发现了两种含囊泡的轴突类型；含小颗粒囊泡的轴突和含小无颗粒囊泡的轴突。在上皮中，观察到两种纤维类型；一种仅含线粒体，另一种既有无颗粒囊泡又有线粒体。
