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使用新型蛋壳改性玻璃离子水门汀对各种穿孔修复材料的封闭能力进行分光光度法比较评估。

A Spectrophotometric comparative evaluation of the sealing ability of various perforation repair materials with a novel eggshell modified GIC.

作者信息

Abraham Sathish, Chandwani Ekta Dharmendra, Nagmode Pradnya, Lokhande Nitin, Badgujar Manish Bhaskar, Diggikar Kshitija

机构信息

Department of Conservative Dentistry and Endodontics, SMBT Dental College and Hospital, Ahmednagar, Maharashtra, India.

出版信息

J Conserv Dent Endod. 2023 Nov-Dec;26(6):697-701. doi: 10.4103/JCDE.JCDE_193_23. Epub 2023 Nov 22.

Abstract

OBJECTIVES

The objective of this study was to evaluate and compare the sealing ability of mineral trioxide aggregate (MTA), Biodentine, glass ionomer cement, and glass ionomer cement modified with Chicken Eggshell Powder when used as furcation perforation repair material.

MATERIALS AND METHODS

In the present study, 80 human lower first molars were used. Collected teeth had no caries or restoration, and none had fused roots. Every molar had an endodontic access cavity made utilizing a high-speed, long-shank round bur for the initial entry and an Endo-Z for lateral extension and finishing the cavity walls. Each canal's orifice was covered with a temporary filling material. The pulpal floor and cavity walls of every molar were thoroughly coated with two successive coats of clear nail polish. A significant perforation was made between the orifices to the furcation area using a high-speed long shank round bur #4. The perforation centered between the mesial and distal orifice. They were divided into four experimental groups: Group I: 20 molars were repaired using MTA, Group II: 20 molars with Biodentine, Group III: 20 molars were repaired with glass ionomer cement, and Group IV was repaired using glass ionomer cement modified with 7% chicken eggshell powder. Moist cotton pellets were placed over the repair materials, and molars were kept in 100% humidity for 24 h to allow materials to set. Then, according to each group, molars were put in Petri dishes. Methylene blue dye was applied inside the access cavity of all samples for 24 h. Molars were placed under running tap water for 30 min to remove all residues of methylene blue, and then varnish was removed with a Parker blade #15 and polishing discs. Molars were placed in vials containing 1 mL of concentrated (65 wt%) nitric acid for 3 days. Vials were centrifuged at 14,000 rpm for 5 min. The supernatant from each sample was transferred in a quantity of 200 L to a 96-well plate. Sample absorbance was read by an automatic microplate spectrophotometer at 550 nm using concentrated nitric acid as a blank. Statistical analysis was performed using one-way analysis of variance. When the analysis of variance test was significant, the pairwise comparison of the means was done using a Duncan test. The significance level was set at < 0.05. Statistical analysis was performed with SPSS 14.0 (SPSS Inc., Chicago, IL, USA) for Windows (Microsoft, Redmond, WA, USA).

RESULTS

The highest dye absorbance was seen in Group III, followed by Groups IV, II, and I.

CONCLUSION

Within the limitations of study it was concluded that maximum sealing ability was seen in Biodentine followed by MTA, Glass Ionomer Cement modified with 7% Chicken Eggshell powder and Glass Ionomer Cement.

摘要

目的

本研究的目的是评估和比较矿物三氧化物凝聚体(MTA)、生物活性玻璃陶瓷(Biodentine)、玻璃离子水门汀以及用鸡蛋壳粉改性的玻璃离子水门汀作为根分叉穿孔修复材料时的封闭能力。

材料与方法

在本研究中,使用了80颗人类下颌第一磨牙。收集的牙齿无龋坏或修复体,且无融合根。每颗磨牙均使用高速长柄圆钻制作牙髓腔入口,并用Endo-Z钻进行侧向扩展并修整腔壁。每个根管口均用临时充填材料覆盖。每颗磨牙的髓室底和腔壁均先后涂覆两层透明指甲油。使用高速长柄4号圆钻在根管口之间向根分叉区域制作一个明显的穿孔。穿孔位于近中根管口和远中根管口之间的中心位置。将它们分为四个实验组:第一组:20颗磨牙用MTA修复;第二组:20颗磨牙用生物活性玻璃陶瓷修复;第三组:20颗磨牙用玻璃离子水门汀修复;第四组用7%鸡蛋壳粉改性的玻璃离子水门汀修复。在修复材料上放置湿棉球,磨牙在100%湿度下保存24小时以使材料凝固。然后,根据每组情况,将磨牙放入培养皿中。在所有样本的髓腔入口内施加亚甲基蓝染料24小时。将磨牙置于流水下冲洗30分钟以去除所有亚甲基蓝残留物,然后用15号派克刀片和抛光盘去除指甲油。将磨牙放入含有1 mL浓硝酸(65 wt%)的小瓶中3天。将小瓶以14,000 rpm离心5分钟。将每个样本的上清液取200 μL转移至96孔板中。使用自动酶标仪在550 nm处读取样本吸光度,以浓硝酸作为空白对照。采用单因素方差分析进行统计分析。当方差分析检验具有显著性时,使用邓肯检验进行均值的两两比较。显著性水平设定为P < 0.05。使用SPSS 14.0(SPSS公司,美国伊利诺伊州芝加哥)对Windows(微软公司,美国华盛顿州雷德蒙德)进行统计分析。

结果

第三组的染料吸光度最高,其次是第四组、第二组和第一组。

结论

在本研究的局限性范围内,得出的结论是,生物活性玻璃陶瓷的封闭能力最强,其次是MTA、7%鸡蛋壳粉改性的玻璃离子水门汀和玻璃离子水门汀。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4471/10823989/3843cf608a54/JCDE-26-697-g001.jpg

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