Wang Kang, Li Guanzong, Yang Zhi, Yang Fumei, Sun Yulin, Duan Gang, Sun Wang, Zhou Ke, He Jun, Dai Feiyan
College of Veterinary Medicine, Yunnan Agricultural University, Kunming, China.
College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Front Microbiol. 2024 Jan 16;14:1292082. doi: 10.3389/fmicb.2023.1292082. eCollection 2023.
Compound Chinese medicine (F1) is a traditional prescription in Chinese medicine that is commonly used to treat spleen deficiency diarrhea (SDD). It has demonstrated remarkable effectiveness in clinical practice. However, the precise mechanism by which it exerts its antidiarrheal effect is still unclear. This study aimed at investigating the antidiarrheal efficacy and mechanism of F1 on senna-induced secretory diarrhea (SDD). Senna was utilized to induce the development of a mouse model of senna-induced secretory diarrhea (SDD) in order to observe the rate of diarrhea, diarrhea index, blood biochemistry, and histopathological changes in the small intestine. Additionally, the levels of sodium and hydrogen exchange protein 3 (NHE3) and short-chain fatty acids (SCFAs) were determined using enzyme-linked immunosorbent assay (ELISA). The impact of F1 on the senna-induced SDD mouse models was evaluated by monitoring changes in the gut microbiota through 16S rRNA (V3-V4) sequencing. The results demonstrated that F1, a traditional Chinese medicine, effectively increased the body weight of SDD mice and reduced the incidence of diarrhea and diarrhea index. Additionally, F1 restored liver and kidney function, reduced the infiltration of inflammatory cells in intestinal tissue, and promoted the growth of intestinal villi. Furthermore, F1 was found to enhance the expression of NHE3 and SCFAs. It also increased the abundance of Firmicutes and Lactobacillus species, while decreasing the abundance of Proteobacteria and Shigella.
复方中药（F1）是中医常用的治疗脾虚泄泻（SDD）的传统方剂。它在临床实践中已显示出显著疗效。然而，其发挥止泻作用的确切机制仍不清楚。本研究旨在探讨F1对番泻叶诱导的分泌性腹泻（SDD）的止泻疗效及机制。利用番泻叶诱导建立番泻叶诱导的分泌性腹泻（SDD）小鼠模型，观察腹泻率、腹泻指数、血液生化指标以及小肠组织病理学变化。此外，采用酶联免疫吸附测定（ELISA）法测定钠氢交换蛋白3（NHE3）和短链脂肪酸（SCFAs）水平。通过16S rRNA（V3-V4）测序监测肠道微生物群的变化，评估F1对番泻叶诱导的SDD小鼠模型的影响。结果表明，中药F1能有效增加SDD小鼠体重，降低腹泻发生率和腹泻指数。此外，F1还能恢复肝肾功能，减少肠道组织中炎性细胞浸润，促进肠绒毛生长。此外，还发现F1能增强NHE3和SCFAs的表达。它还增加了厚壁菌门和乳酸杆菌属的丰度，同时降低了变形菌门和志贺氏菌属的丰度。
