Tran Dinh Minh, Nguyen Thi Huyen
Institute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot, Dak Lak 630000, Vietnam.
Data Brief. 2024 Jan 9;52:110039. doi: 10.1016/j.dib.2024.110039. eCollection 2024 Feb.
Vietnam is currently one of the largest producers and exporters of cashew nuts in the world. Cashew ( L.) is one of the main industrial crops cultivated in Dak Lak Province of Vietnam. Comprehending the endophytic bacteria of this plant, a new biofertilizer for sustainable cashew nut production can be progressed. In this report, the cashew root sample was collected from cashew fields in 2021 in Dak Lak. The DNeasy Powersoil kit was used to extract the genomic DNA of endophytic bacteria from the root sample. The 16S rRNA genes (V1-V9 regions) were amplified by PCR, and libraries of amplicons were prepared using the Swift amplicon 16S plus ITS panel kit. The Illumina MiSeq platform was applied to sequence amplicon libraries using 16S rRNA metagenomics. Taxonomic analyses showed that Gammaproteobacteria (38.77 %) and Alphaproteobacteria (37.76 %) were the predominant classes among the endophytic bacteria. Functional analyses revealed that biosynthesis (72.78 %) was the primary function of the endophytic bacterial community. Raw sequences (Fastq files) have been deposited in Mendeley Data [1]. The obtained data provide insight into the endophytic bacterial community of cashews cultivated in Dak Lak Province of Vietnam. The data are valuable for further developing a new biofertilizer for cashew nut production using endophytic bacteria. Ours is the first report about endophytic bacterial communities of cashews cultivated in this province as well as the Central Highlands of Vietnam.
越南是目前世界上最大的腰果生产国和出口国之一。腰果(Anacardium occidentale L.)是越南多乐省种植的主要经济作物之一。了解这种植物的内生细菌,有助于开发一种用于可持续腰果生产的新型生物肥料。在本报告中,2021年从越南多乐省的腰果种植园采集了腰果根样本。使用DNeasy Powersoil试剂盒从根样本中提取内生细菌的基因组DNA。通过PCR扩增16S rRNA基因(V1-V9区域),并使用Swift扩增子16S加ITS分析试剂盒制备扩增子文库。应用Illumina MiSeq平台通过16S rRNA宏基因组学对扩增子文库进行测序。分类学分析表明,γ-变形菌纲(38.77%)和α-变形菌纲(37.76%)是内生细菌中的主要菌纲。功能分析显示,生物合成(72.78%)是内生细菌群落的主要功能。原始序列(Fastq文件)已存入Mendeley Data [1]。所获得的数据为了解越南多乐省种植的腰果的内生细菌群落提供了线索。这些数据对于利用内生细菌进一步开发用于腰果生产的新型生物肥料具有重要价值。我们的研究是关于该省以及越南中部高地种植的腰果内生细菌群落的首份报告。