Medicine Department, Microbiology Area, School of Medicine, Complutense University of Madrid, Madrid, Spain.
Nursing Department, Faculty of Nursing, Physiotherapy and Podiatry, Universidad Complutense de Madrid, Madrid, Spain.
J Med Virol. 2024 Feb;96(2):e29431. doi: 10.1002/jmv.29431.
The aim of the study was to determine the presence of human papillomavirus (HPV) in patients with intractable plantar keratosis (IPK) by comparing the histopathological findings of biopsies. A prospective, observational, and concordance study was carried out. Three different specimens were taken from each IPK. A first punch was sent for histopathological examination, and a second punch and a superficial skin scraping were both sent for HPV polymerase chain reaction (PCR) and type determination. A total of 51 patients were included. From the histopathological examination, it was determined that 35 (68.6%) samples were diagnosed as warts and 16 (31.3%) as keratosis. However, the presence of HPV was confirmed by PCR in 49 (96.1%) and in 42 (82.4%) samples obtained by punch and superficial scraping, respectively. In the 49 PCR-positive samples, the most common HPV types were HPV1, HPV2, HPV27, HPV57, and HPV65, accounting for 81.6% of the samples. In conclusion, this study demonstrates that HPV infection and IPK lesions are very closely related. Although we cannot confirm that HPV is the cause of the development of IPK, the high prevalence of HPV observed in these lesions calls for a change to the procedures for managing IPK.
本研究旨在通过比较活检的组织病理学发现来确定顽固性足底角化病（IPK）患者是否存在人乳头瘤病毒（HPV）。进行了一项前瞻性、观察性和一致性研究。从每个 IPK 中采集了三个不同的标本。第一个打孔器用于组织病理学检查，第二个打孔器和浅层皮肤刮片都用于 HPV 聚合酶链反应（PCR）和类型确定。共纳入 51 名患者。通过组织病理学检查，确定 35 个（68.6%）样本被诊断为疣，16 个（31.3%）为角化病。然而，通过 PCR 在 49 个打孔器和浅层刮片获得的样本中分别证实了 49（96.1%）和 42（82.4%）样本中存在 HPV。在 49 个 PCR 阳性样本中，最常见的 HPV 类型是 HPV1、HPV2、HPV27、HPV57 和 HPV65，占样本的 81.6%。综上所述，本研究表明 HPV 感染与 IPK 病变密切相关。尽管我们不能确认 HPV 是 IPK 发展的原因，但在这些病变中观察到的 HPV 高流行率呼吁改变 IPK 的治疗程序。
