Department of Coaching Education, Sivas Cumhuriyet University, Sivas, Turkey.
Department of Physical Education and Sports Teaching, Fırat University, Elaziğ, Turkey.
Biotech Histochem. 2024 Feb;99(2):69-75. doi: 10.1080/10520295.2024.2305113. Epub 2024 Jan 31.
Myonectin is a hormone that is produced mainly by skeletal muscle. We investigated the effects of exercise and energy drink (ED) administration on myonectin expression in skeletal muscle, liver and kidney tissue in rats; myonectin is produced by all three tissues. We used 28 male albino rats in four groups: untreated control (C), exercise (E), energy drink (ED) and exercise + energy drink (E + ED). The E and E + ED groups were exercised using a treadmill for 4 weeks. We also administered 3.5 ml/kg/day ED during week 1, 7 ml/kg/day during week 2 and 10 ml/kg/day during weeks 3 and 4 in the E and E + ED groups. We used ELISA to measure the levels of myonectin in skeletal muscle, liver and kidney tissues. We used immunohistochemical staining to investigate the localization and intensity of myonectin in these tissues. The amount of myonectin in skeletal muscle tissue was increased significantly in all experimental groups compared to group C. The amount of myonectin in the ED group was significantly greater than group E. No significant difference was observed in liver tissue; however, the amount of myonectin in the liver of group C was the greatest among all groups. The amount of myonectin in kidney tissue exhibited no significant difference among groups. Consumption of ED during exercise increased the amount of myonectin in kidney and skeletal muscle tissues and decreased it in liver tissue. We suggest that consumption of ED might adapt metabolism to incresed exercise by controling synthesis of myonectin in liver, kidney and skeletal muscle.
肌联蛋白是一种主要由骨骼肌产生的激素。我们研究了运动和能量饮料（ED）给药对大鼠骨骼肌、肝脏和肾脏组织中肌联蛋白表达的影响；所有三种组织都产生肌联蛋白。我们使用 28 只雄性白化大鼠分为四组：未处理对照组（C）、运动组（E）、能量饮料组（ED）和运动+能量饮料组（E+ED）。E 和 E+ED 组使用跑步机进行 4 周的运动。在 E 和 E+ED 组中，我们在第 1 周给予 3.5 ml/kg/天的 ED，在第 2 周给予 7 ml/kg/天，在第 3 和第 4 周给予 10 ml/kg/天。我们使用 ELISA 测量骨骼肌、肝脏和肾脏组织中肌联蛋白的水平。我们使用免疫组织化学染色来研究这些组织中肌联蛋白的定位和强度。与 C 组相比，所有实验组骨骼肌组织中的肌联蛋白含量均显著增加。ED 组的肌联蛋白含量明显高于 E 组。在肝脏组织中未观察到显著差异；然而，C 组肝脏中的肌联蛋白含量在所有组中最高。在肾脏组织中，肌联蛋白含量在各组之间无显著差异。运动期间摄入 ED 增加了肾脏和骨骼肌组织中肌联蛋白的含量，减少了肝脏组织中肌联蛋白的含量。我们认为，ED 的摄入可能通过控制肝脏、肾脏和骨骼肌中肌联蛋白的合成来适应运动引起的代谢增加。
