低浓度尿白蛋白的酶联免疫吸附测定
Enzyme-linked immunosorbent assay for urinary albumin at low concentrations.
作者信息
Krämer B K, Jesse U, Ress K M, Schmülling R M, Risler T
出版信息
Clin Chem. 1987 Apr;33(4):609-11.
We describe an enzyme-linked immunosorbent assay (ELISA) for urinary albumin. It requires only commercially available reagents, can detect as little as 16 micrograms of albumin per liter, and analytical recovery ranges from 92 to 116%. The assay is simple, rapid, and inexpensive. Albumin excretion was 6.2 (SD 4.1) mg/24 h in healthy subjects (n = 40), 14.7 (SD 7.2) mg/24 h in albumin-test-strip-negative Type I diabetics (n = 11), and 19.7 (SD 16.2) mg/24 h in patients with essential hypertension (n = 12).
我们描述了一种用于检测尿白蛋白的酶联免疫吸附测定法（ELISA）。该方法仅需使用市售试剂，每升可检测低至16微克的白蛋白，分析回收率在92%至116%之间。该测定法简便、快速且成本低廉。健康受试者（n = 40）的白蛋白排泄量为6.2（标准差4.1）毫克/24小时，白蛋白试纸条检测为阴性的I型糖尿病患者（n = 11）为14.7（标准差7.2）毫克/24小时，原发性高血压患者（n = 12）为19.7（标准差16.2）毫克/24小时。
Zotero 插件