Mohamed A, Wilkin T, Leatherdale B, Davies R
J Immunol Methods. 1984 Nov 16;74(1):17-22. doi: 10.1016/0022-1759(84)90362-4.
Interest in early albuminuria ('microalbuminuria') has intensified recently, particularly in the area of diabetes. We describe a sandwich type enzyme-linked immunoassay for urinary albumin and compare it with a conventional radioimmunoassay (RIA) using the same commercial antiserum. The assay ranges for ELISA and RIA were 7.5-500 micrograms/l and 20-5000 micrograms/l respectively and the corresponding sensitivities were 1.5 ng and 2.0 ng. The correlation coefficient between ELISA and RIA in the measurement of albumin in overnight urine samples from 74 children was r = 0.89 (P less than 0.001). ELISA has several practical advantages over radioimmunoassay, and we believe it has the potential to replace RIA in the screening and study of albuminuria.
近年来，人们对早期蛋白尿（“微量白蛋白尿”）的关注日益增加，尤其是在糖尿病领域。我们描述了一种用于检测尿白蛋白的夹心型酶联免疫吸附测定法，并将其与使用相同商业抗血清的传统放射免疫测定法（RIA）进行比较。酶联免疫吸附测定法（ELISA）和放射免疫测定法（RIA）的检测范围分别为7.5 - 500微克/升和20 - 5000微克/升，相应的灵敏度分别为1.5纳克和2.0纳克。在对74名儿童过夜尿液样本中的白蛋白进行测量时，ELISA和RIA之间的相关系数r = 0.89（P < 0.001）。与放射免疫测定法相比，酶联免疫吸附测定法具有几个实际优势，我们认为它有潜力在蛋白尿的筛查和研究中取代放射免疫测定法。
