Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, People's Republic of China.
Jiangsu Province Key Laboratory of Green Biomass-Based Fuels and Chemicals, Nanjing, 210037, People's Republic of China.
World J Microbiol Biotechnol. 2024 Jan 31;40(3):84. doi: 10.1007/s11274-023-03882-z.
Arabinoxylans (AXs) are hemicellulosic polysaccharides consisting of a linear backbone of β-1,4-linked xylose residues branched by high content of α-L-arabinofuranosyl (Araf) residues along with other side-chain substituents, and are abundantly found in various agricultural crops especially cereals. The efficient bioconversion of AXs into monosaccharides, oligosaccharides and/or other chemicals depends on the synergism of main-chain enzymes and de-branching enzymes. Exo-α-L-arabinofuranosidases (ABFs) catalyze the hydrolysis of terminal non-reducing α-1,2-, α-1,3- or α-1,5- linked α-L-Araf residues from arabinose-substituted polysaccharides or oligosaccharides. ABFs are critically de-branching enzymes in bioconversion of agricultural biomass, and have received special attention due to their application potentials in biotechnological industries. In recent years, the researches on microbial ABFs have developed quickly in the aspects of the gene mining, properties of novel members, catalytic mechanisms, methodologies, and application technologies. In this review, we systematically summarize the latest advances in microbial ABFs, and discuss the future perspectives of the enzyme research.
阿拉伯木聚糖（AXs）是一种半纤维素多糖，由β-1,4 连接的木糖残基组成的线性主链，通过高含量的α-L-阿拉伯呋喃糖基（Araf）残基沿主链分支，以及其他侧链取代基。AXs 在各种农业作物中含量丰富，特别是谷物。AXs 有效地转化为单糖、寡糖和/或其他化学品，取决于主链酶和去支链酶的协同作用。外切-α-L-阿拉伯呋喃糖苷酶（ABFs）催化从阿拉伯糖取代的多糖或寡糖中水解末端非还原的α-1,2-、α-1,3-或α-1,5-连接的α-L-Araf 残基。ABFs 是农业生物质生物转化中关键的去支链酶，由于其在生物技术产业中的应用潜力，受到了特别关注。近年来，微生物 ABFs 的研究在基因挖掘、新型成员的特性、催化机制、方法学和应用技术等方面取得了快速发展。在本文中，我们系统地总结了微生物 ABFs 的最新进展，并讨论了该酶研究的未来展望。
