Ju Ye-Rin, Im Su Been, Jung Da Eun, Son Min Jeong, Park Chan-Young, Jeon Min Ho, Hwang Ju Hee, Lee Soo Jung, Kim Tae-Jip
Division of Animal, Horticultural and Food Sciences, Graduate School of Chungbuk National University, Cheongju 28644, Republic of Korea.
Department of Food Science and Biotechnology, Chungbuk National University, Cheongju 28644, Republic of Korea.
J Microbiol Biotechnol. 2025 Jan 13;35:e2412052. doi: 10.4014/jmb.2412.12052.
There is growing interest in pentose-based prebiotic oligosaccharides as alternatives to traditional hexose-based prebiotics. Among these, arabino-oligosaccharides (AOS), derived from the enzymatic hydrolysis of arabinan polymers, have gained significant attention. AOS can selectively stimulate the growth of beneficial gut bacteria, including and species, and contribute to health-benefit functions such as blood sugar control, positioning AOS as a promising synbiotic candidate. For the industrial production of AOS, the development of efficient enzymatic processes is essential, with exo- and endo-1,5-α-L-arabinanases (exo- and endo-ABNs) playing a crucial catalytic role. Most ABNs belong to the glycoside hydrolase (GH) family 43, characterized by a five-bladed β-propeller fold structure. These enzymes hydrolyze internal α-1,5-L-arabinofuranosidic linkages, producing AOS with varying degrees of polymerization. Some ABNs GH43 were known to exhibit exo-type hydrolytic modes of action, producing specific AOS products such as arabinotriose. Additionally, exo-ABNs from GH93, which feature a six-bladed β-propeller fold, exclusively release arabinobiose through their exo-type catalytic mechanism. This review represents the first comprehensive analysis of exo- and endo-ABNs, offering scientific insights into their biotechnological potential for AOS production. It systematically compares enzyme classification, structural differences, catalytic mechanisms, paving the way for innovative applications in health, food, and pharmaceutical industries.
作为传统基于己糖的益生元的替代品，基于戊糖的益生元低聚糖越来越受到关注。其中，由阿拉伯聚糖聚合物酶解产生的阿拉伯低聚糖（AOS）受到了广泛关注。AOS可以选择性地刺激有益肠道细菌的生长，包括[具体细菌种类1]和[具体细菌种类2]等，并有助于实现血糖控制等健康益处功能，这使得AOS成为一种有前途的合生元候选物。对于AOS的工业化生产，高效酶促工艺的开发至关重要，外切和内切1,5-α-L-阿拉伯聚糖酶（外切和内切ABN）起着关键的催化作用。大多数ABN属于糖苷水解酶（GH）家族43，其特征是具有五叶β-螺旋桨折叠结构。这些酶水解内部的α-1,5-L-阿拉伯呋喃糖苷键，产生不同聚合度的AOS。已知一些GH43家族的ABN表现出外切型水解作用模式，产生特定的AOS产物，如阿拉伯三糖。此外，来自GH93家族的外切ABN具有六叶β-螺旋桨折叠结构，通过其外切型催化机制专门释放阿拉伯二糖。本综述首次对外切和内切ABN进行了全面分析，为其在AOS生产中的生物技术潜力提供了科学见解。它系统地比较了酶的分类、结构差异、催化机制，为在健康、食品和制药行业的创新应用铺平了道路。
