使用上转换纳米粒子对秀丽隐杆线虫中的神经元和运动行为进行近红外光遗传学操作的方案。

Protocol for near-infrared optogenetics manipulation of neurons and motor behavior in C. elegans using emissive upconversion nanoparticles.

机构信息

College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.

College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China; National Engineering Research Center for Nanomedicine, Huazhong University of Science and Technology, Wuhan 430074, China.

出版信息

STAR Protoc. 2024 Mar 15;5(1):102858. doi: 10.1016/j.xpro.2024.102858. Epub 2024 Jan 30.

DOI:10.1016/j.xpro.2024.102858

PMID:38294907

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10846378/

Abstract

In deep tissue, optogenetics faces limitations with visible light. Here, we present a protocol for near-infrared (NIR) optogenetics manipulation of neurons and motor behavior in Caenorhabditis elegans using emissive upconversion nanoparticles (UCNPs). We describe steps for synthesizing and modifying UCNPs. We then detail procedures for regulating neurons using these UCNPs in the model organism C. elegans. Using NIR light allows for superior tissue penetration to manipulate neuronal activities and locomotion behavior. For complete details on the use and execution of this protocol, please refer to Guo et al., Ao et al., and Zhang et al..

摘要

在深层组织中，可见光是光遗传学的限制因素。在这里，我们提出了一种使用上转换纳米粒子（UCNPs）对秀丽隐杆线虫中的神经元和运动行为进行近红外（NIR）光遗传学操纵的方案。我们描述了合成和修饰 UCNPs 的步骤。然后，我们详细介绍了在模式生物秀丽隐杆线虫中使用这些 UCNPs 来调节神经元的程序。使用近红外光可以更好地穿透组织，从而操纵神经元活动和运动行为。如需了解此方案的使用和执行的完整详细信息，请参阅 Guo 等人、Ao 等人和 Zhang 等人的研究。