Institute of Research and Development, Duy Tan University, Da Nang, Vietnam; School of Engineering & Technology, Duy Tan University, Da Nang, Vietnam.
Department of Physics and Astronomy, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia.
Environ Res. 2024 May 1;248:118348. doi: 10.1016/j.envres.2024.118348. Epub 2024 Jan 29.
The antimicrobial, antidiabetic, and anti-inflammatory activities efficiency of Aerva lanata plant extracts (aqueous (Aqu-E), acetone (Ace-E), and ethanol (Eth-E)) were investigated in this study. Furthermore, the active molecules exist in the crude extract were characterized by UV-Visible spectrophotometer, Fourier transform infrared (FTIR), High-performance liquid chromatography (HPLC), and Gas Chromatography-Mass Spectrometry (GC-MS) analyses. The preliminary phytochemical study revealed that the Ace-E restrain more phytochemicals like alkaloids, saponins, anthraquinone, tannins, phenolics, flavonoids, glycosides, terpenoids, amino acid, steroids, protein, coumarin, as well as quinine than Aqu-E and Eth-E. Accordingly to this Ace-E showed considerable antimicrobial activity as the follows: for bacteria S. aureus > E. coli > K. pneumoniae > P. aeruginosa > B. subtilis and for fungi T. viride > A.flavus > C. albicans > A.niger at 30 mg ml concentration. Similarly, Ace-E showed considerable antidiabetic (α-amylase: 71.7 % and α-glucosidase: 70.1 %) and moderate anti-inflammatory (59 % and 49.8 %) activities. The spectral and chromatogram studies confirmed that the Ace-E have pharmaceutically valuable bioactive molecules such as (Nbutyl)-octadecane, propynoic acid, neophytadiene, and 5,14-di (N-butyl)-octadecane. These findings suggest that Ace-E from A. lanata can be used to purify additional bioactive substances and conduct individual compound-based biomedical application research.
本研究考察了 Aerva lanata 植物提取物（水提物（Aqu-E）、丙酮提物（Ace-E）和乙醇提物（Eth-E））的抗菌、抗糖尿病和抗炎活性。此外，还通过紫外可见分光光度计、傅里叶变换红外光谱（FTIR）、高效液相色谱（HPLC）和气相色谱-质谱联用（GC-MS）分析对粗提取物中的活性分子进行了表征。初步的植物化学研究表明，Ace-E 比 Aqu-E 和 Eth-E 含有更多的生物碱、皂苷、蒽醌、单宁、酚类、类黄酮、糖苷、萜类、氨基酸、甾体、蛋白质、香豆素和奎宁等植物化学物质。因此，Ace-E 表现出相当的抗菌活性，对细菌的抑制作用依次为：金黄色葡萄球菌>大肠杆菌>肺炎克雷伯菌>铜绿假单胞菌>枯草芽孢杆菌；对真菌的抑制作用依次为：绿色木霉>黄曲霉>白色念珠菌>黑曲霉，浓度均为 30mg/ml。同样，Ace-E 表现出相当的抗糖尿病（α-淀粉酶：71.7%和α-葡萄糖苷酶：70.1%）和适度的抗炎（59%和 49.8%）活性。光谱和色谱研究证实，Ace-E 含有具有药用价值的生物活性分子，如（正丁基）-十八烷、丙炔酸、新 Phytadiene 和 5,14-双（正丁基）-十八烷。这些发现表明，A. lanata 的 Ace-E 可用于纯化其他生物活性物质，并进行基于单个化合物的生物医学应用研究。
