Shanghai Jiading District Center for Disease Control and Prevention, Shanghai 201800, China.
J Food Prot. 2024 Mar;87(3):100234. doi: 10.1016/j.jfp.2024.100234. Epub 2024 Feb 1.
To investigate the distribution, contamination status, and antibiotic resistance of Listeria monocytogenes in four types of retail poultry meat, including chicken, duck, goose, and pigeon, sold in Jiading District, Shanghai, a total of 236 retail poultry meat samples were collected, and L. monocytogenes isolates were obtained for identification and antibiotic susceptibility testing against 14 common antibiotics. Forty-one L. monocytogenes isolates were detected from the 236 retail poultry meat samples, with detection rates of 24.47%, 19.44%, 14.75%, and 4.44% in chicken, goose, duck, and pigeon meat, respectively. Among refrigerated, frozen, and room temperature samples, refrigerated poultry had the highest detection rate at 25.40%, while frozen poultry had the lowest at 13.33%. The detection rate of L. monocytogenes in chicken meat differed significantly between the storage temperatures, while no significant differences were found for other poultry types. No significant differences in detection rates were observed between different retail locations or packaging methods. Isolates exhibited complete resistance to cefoxitin (FOX) and increasing resistance over time to tetracycline (TET) and clindamycin (CLI), while low levels of resistance were found for penicillin (PEN), oxacillin (OXA), and erythromycin (ERY). Resistance to ERY and TET suggests the potential for multidrug resistance. Significant differences in antibiotic resistance profiles were observed among L. monocytogenes from the various poultry types. In summary, contamination status and antibiotic resistance profiles differed among retail chicken, duck, goose, and pigeon meat sold and the resistance rate of strains continues to increase in Jiading District, Shanghai. Targeted control measures should be implemented to reduce the emergence of resistant strains, as retail conditions had minimal impact on L. monocytogenes prevalence in poultry meat.
为了研究上海市嘉定区四种零售禽类肉（鸡肉、鸭肉、鹅肉和鸽肉）中李斯特菌的分布、污染状况和抗生素耐药性，共采集了 236 份零售禽类肉样本，分离出李斯特菌进行鉴定和对 14 种常见抗生素的药敏试验。从 236 份零售禽类肉样本中检测到 41 株李斯特菌，鸡、鹅、鸭和鸽肉的检出率分别为 24.47%、19.44%、14.75%和 4.44%。在冷藏、冷冻和室温样本中，冷藏禽类的检出率最高，为 25.40%，而冷冻禽类的检出率最低，为 13.33%。鸡肉中李斯特菌的检出率在不同贮藏温度下差异显著，而其他禽类类型则无显著差异。不同零售地点或包装方式的检出率无显著差异。分离株对头孢西丁（FOX）表现出完全耐药，对四环素（TET）和克林霉素（CLI）的耐药性随时间推移而逐渐增加，而对青霉素（PEN）、苯唑西林（OXA）和红霉素（ERY）的耐药性较低。对 ERY 和 TET 的耐药性提示可能存在多药耐药性。不同禽类来源的李斯特菌的抗生素耐药谱存在显著差异。综上所述，零售的鸡肉、鸭肉、鹅肉和鸽肉的污染状况和抗生素耐药谱不同，且在上海市嘉定区，菌株的耐药率持续上升。应采取有针对性的控制措施，以减少耐药菌株的出现，因为零售条件对禽类肉中李斯特菌的流行影响较小。
