Ganguly T, Pakrashi A, Pal A K
Contraception. 1986 Dec;34(6):625-37. doi: 10.1016/s0010-7824(86)80018-x.
Aristolic acid (AA), obtained from Aristolochia indica Linn, disrupted nidation in mice when administered on Day 1 of pregnancy. The implantation inhibiting effect of the compound was assessed with respect to certain parameters which are characteristics of early pregnancy, such as tubal transport of ova into the uterus, hyperpermeability of the endometrial capillaries, increase in uterine weight and total protein content, endometrial bed preparation and changes in uterine phosphatase enzymes during Days 4-6 of pregnancy. The compound did not affect tubal transport of eggs, but the uterine blue reaction, caused by extravasation of the dye, pontamine blue, at future implantation sites was inhibited significantly in treated mice. Histological picture of the uterus revealed AA-induced impairment of development (i.e. decidualization) and reconciled with decreases found in uterine weight and its total protein contents in treated animals. In control untreated mice, specific uterine alkaline phosphatase (ALP) activity increased significantly from Days 4 through 6 of pregnancy, but this was prevented in treated mice. On the other hand, specific uterine acid phosphatase (AP) activity was high on Day 5, while in treated mice uterine AP activity remained low during Days 4 and 5 and increased significantly thereafter. It was inferred that AA interferes with steroidal conditioning of the uterus and renders it hostile to ovum implantation.
从印度马兜铃中提取的马兜铃酸（AA），在妊娠第1天给药时会干扰小鼠着床。根据某些早孕特征参数评估了该化合物的着床抑制作用，这些参数包括卵子从输卵管向子宫的运输、子宫内膜毛细血管的高通透性、子宫重量和总蛋白含量的增加、子宫内膜床的准备以及妊娠第4 - 6天子宫磷酸酶的变化。该化合物不影响卵子的输卵管运输，但在处理过的小鼠中，由染料滂胺蓝在未来着床部位外渗引起的子宫蓝色反应受到显著抑制。子宫的组织学图像显示，AA导致发育（即蜕膜化）受损，这与处理过的动物子宫重量及其总蛋白含量的降低一致。在未处理的对照小鼠中，妊娠第4天至第6天子宫特异性碱性磷酸酶（ALP）活性显著增加，但在处理过的小鼠中这种情况得到了阻止。另一方面，子宫特异性酸性磷酸酶（AP）活性在第5天高，而在处理过的小鼠中，子宫AP活性在第4天和第5天保持较低水平，此后显著增加。据推断，AA会干扰子宫的甾体调节，使其对卵子着床产生敌意。
