Wu Tong, Chen Zhihui, Liu Youwen, Sheng Qinghong, Lu Yuangang, Wang Jiming, He Chongjun, Shi Yaoyao
Opt Express. 2024 Jan 15;32(2):2774-2785. doi: 10.1364/OE.512263.
Lissajous micro scanners are very attractive in compact laser scanning applications for biomedical endoscopic imaging, such as confocal microscopy, endomicroscopy or optical coherence tomography. The scanning frequencies have a very important effect on the quality of the resulting Lissajous scanning imaging. In this paper, we propose a frequency selection rule for high definition and high frame-rate (HDHF) Lissajous scanning imaging, by deriving the relationship among the scanning field of view (FOV), actuation frequencies and pixel size based on the characteristics of the scanning trajectory. The minimum sampling rate based on the proposed frequency selection rule is further discussed. We report a lead zirconate titanate piezoelectric ceramic (PZT) based Lissajous fiber scanner to achieve HDHF Lissajous scanning imaging. Based on the frequency selection rule, different frequency combinations are calculated, under which the Lissajous fiber scanner can work at the frame rate (FR) of 10 Hz, 20 Hz, 40 Hz and 52 Hz. The trajectory evolution of the Lissajous scanning at the frame rate of 10 Hz has been obtained to verify the applicability of the proposed rule. The measured resolution of the scanner is 50.8 lp/mm at the unit optical magnification, and the measured FOV at the FR of 10 Hz and 40 Hz are 1.620 mm ×1.095 mm and 0.405 mm ×0.27 mm, respectively. HDHF Lissajous scanning images of the customized spatial varying binary pattern are obtained and reconstructed at the FR of 10 Hz and 40 Hz, demonstrating the practicability of the frequency selection rule.
李萨如图形微扫描仪在用于生物医学内镜成像的紧凑型激光扫描应用中非常有吸引力,如共聚焦显微镜、内镜显微镜或光学相干断层扫描。扫描频率对最终的李萨如图形扫描成像质量有非常重要的影响。在本文中,我们基于扫描轨迹的特性,通过推导扫描视野(FOV)、驱动频率和像素大小之间的关系,提出了一种用于高清晰度和高帧率(HDHF)李萨如图形扫描成像的频率选择规则。进一步讨论了基于所提出频率选择规则的最小采样率。我们报道了一种基于锆钛酸铅压电陶瓷(PZT)的李萨如图形光纤扫描仪,以实现HDHF李萨如图形扫描成像。基于频率选择规则,计算了不同的频率组合,在这些组合下,李萨如图形光纤扫描仪可以在10Hz、20Hz、40Hz和52Hz的帧率下工作。已获得10Hz帧率下李萨如图形扫描的轨迹演变,以验证所提出规则的适用性。该扫描仪在单位光学放大倍数下的测量分辨率为50.8 lp/mm,在10Hz和40Hz帧率下的测量视野分别为1.620 mm×1.095 mm和0.405 mm×0.27 mm。在10Hz和40Hz帧率下获得并重建了定制的空间变化二进制图案的HDHF李萨如图形扫描图像,证明了频率选择规则的实用性。