Sakamoto Mizuki, Ishiuchi Takashi
Faculty of Life and Environmental Sciences, University of Yamanashi, Kofu, Yamanashi, Japan.
MicroPubl Biol. 2024 Jan 16;2024. doi: 10.17912/micropub.biology.001108. eCollection 2024.
YY1 plays multifaceted roles in various cell types. We recently reported that YY1 regulates nucleosome organization in early mouse embryos. However, despite the impaired nucleosome organization in the absence of YY1, the transcriptome was minimally affected in eight-cell embryos. We then hypothesized that YY1 might prepare a chromatin environment to regulate gene expression at later stages. To test this possibility, we performed a transcriptome analysis at the morula stage. We found that a substantial number of genes are aberrantly expressed in the absence of YY1. Furthermore, our analysis revealed that YY1 is required for the transcription of LINE-1 retrotransposons.
YY1在多种细胞类型中发挥着多方面的作用。我们最近报道，YY1在小鼠早期胚胎中调节核小体组织。然而，尽管在没有YY1的情况下核小体组织受损，但八细胞胚胎中的转录组受影响最小。然后我们推测，YY1可能会准备一个染色质环境，以便在后期调节基因表达。为了验证这种可能性，我们在桑椹胚阶段进行了转录组分析。我们发现，在没有YY1的情况下，大量基因异常表达。此外，我们的分析表明，YY1是LINE-1逆转录转座子转录所必需的。
