Institute of Biochemistry, Faculty of Biology, Samarkand State University, University Bulvvar, Samarkand, Uzbekistan.
Microsc Res Tech. 2024 Jun;87(6):1201-1209. doi: 10.1002/jemt.24508. Epub 2024 Feb 1.
Microscopic techniques can be applied to solve taxonomic problems in the field of plant systematic and are extremely versatile in nature. This study was focused on the new approaches to visualizing the imaging, tool to cover the micro-structural techniques applied to the pollen study of flowers. The current research was proposed to evaluate microscopic pollen morphological attributes using light and scanning electron microscopy of herbaceous flora from Samarkand, Uzbekistan. A total of 13 herbaceous species, classified into 11 different families were collected, pressed, and identified, and then acetolyzed their pollen to visualize under light and scanning electron microscopy. Herbaceous flora can be characterized by small to very large-sized pollen morphotypes presenting four types of pollen shapes, prolate spheroidal (six species), spheroidal (three species) and prolate and oblate (two species each). The polar diameter and equatorial distance were calculated maximum in Hibiscus syriacus 110.55 and 111.2 μm respectively. Pollen of six different types was found namely tricolporate pollen observed in seven species, tricolpate and pantoporate in two species each, sulcate in Gagea olgae and hexacolpate pollen was examined in Salvia rosmarinus. Exine ornamentation of pollen was examined tectate perforate, verrucate-reticulate, micro-reticulate, reticulate, reticulate-cristatum, gemmate-echinate, echinate-perforate, perforate-striate, rugulate, rugulate-striate, bi-reticulate, reticulate-perforate and perforate-micro-reticulate showing great variations. Exine thickness was noted highest for Rosa canina 2.9 μm and minimum in Punica granatum 0.65 μm. This study of pollen imaging visualization of herbaceous flora contributes to the opportunity for the taxonomic evaluation of and fills knowledge gaps in studies of herbaceous flora identification using classical microscopic taxonomic tools for their accurate identification. RESEARCH HIGHLIGHTS: Pollen in unexplored herbaceous flora of the Samarkand region was studied with light and scanning electron microscopic pollen study. There is a high variation in observed pollen micromorphological characters. Pollen microscopic morphology has important taxonomic value for the identification of herbaceous species.
微观技术可应用于植物系统分类学中的分类问题，且在本质上具有极强的多功能性。本研究专注于新方法，即通过视觉成像来观察工具，涵盖应用于花朵花粉研究的微观结构技术。目前的研究旨在评估乌兹别克斯坦撒马尔罕草本植物区系的微观花粉形态特征，使用光学和扫描电子显微镜。共收集、压制和鉴定了 13 种草本物种，分为 11 个不同科，然后对其花粉进行乙酰化处理，以便在光学和扫描电子显微镜下进行可视化。草本植物区系的花粉形态特征可小至非常大，呈现出四种花粉形状，即长球形（六种）、球形（三种）和长球形和扁球形（每种两种）。Hibiscus syriacus 的极径和赤道距离分别计算为 110.55μm 和 111.2μm。发现六种不同类型的花粉，即七种观察到的三沟花粉、两种各观察到的三沟和多孔花粉、一种观察到的具槽花粉和一种观察到的六沟花粉。花粉纹饰检查显示出纹饰穿孔、疣状-网状、微网状、网状、网状-网脊状、具齿-具刺状、具刺穿孔状、穿孔条纹状、粒状、粒状条纹状、双网状、网状穿孔状和穿孔微网状等多种变化。Rosa canina 的外壁最厚，为 2.9μm，Punica granatum 的外壁最薄，为 0.65μm。本研究对草本植物区系花粉的成像可视化进行了研究，为利用经典的微观分类学工具对草本植物区系进行分类评估提供了机会，并填补了利用这些工具对草本植物区系进行准确鉴定的研究中的知识空白。研究亮点：对撒马尔罕地区未探索的草本植物区系的花粉进行了研究，使用了光学和扫描电子显微镜花粉研究。观察到的花粉微形态特征具有高度变异性。花粉微观形态对草本物种的鉴定具有重要的分类学价值。
