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利用光学显微镜和扫描电子显微镜对巴基斯坦班努地区的 Asteraceae 和 Lamiaceae 科的一些物种进行形态-孢粉学评估。

Morpho-palynological assessment of some species of family Asteraceae and Lamiaceae of District Bannu, Pakistan on the bases of light microscope & scanning electron microscopy.

机构信息

Department of Botany, Abdulwali Khan University, Mardan, Khyber Puhktunkhuwa, Pakistan.

Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan.

出版信息

Microsc Res Tech. 2021 Jun;84(6):1220-1232. doi: 10.1002/jemt.23681. Epub 2021 Feb 1.

Abstract

Pollen micro-morphological features have proven to be helpful for the plant taxonomists in the identification and classification of plants. The aim of this study was to evaluate the palynological features of family Asteraceae and Lamiaceae from flora of District Bannu, Khyber Pakhtunkhwa, Pakistan using both scanning electron microscopy (SEM) and light microscope (LM) for their taxonomic importance. Pollen of seven Asteraceous species belonging to four genera and four Lamiaceae species categorized into four genera were collected from different localities of research area. The present research work provides detailed information of diverse morpho-palynological characters both qualitatively and quantitatively including pollen shape, type, diameter, P/E ratio, exine sculpturing and thickness. Type of pollen in Asteraceae and Lamiaceae was ranged from tricolporate, tricolpate, trizonocolpate and hexazonocolpate. The maximum polar diameter (40.05 μm) and equatorial diameter (37.66 μm) was observed in the Ajuga bracteoosa while minimum polar and equatorial diameter was noted in Isodon rugosus (11.10 μm) and Erigeron canadensis (13.20 μm) respectively. Sculpturing of exine include; echinate, reticulate scabrate, aerolate, reticulate-verrucate, reticulate-scabrate, perforate and reticulate to perforate. Exine thickness was examined maximum 1.50 μm in Helianthus tuberosus, whereas minimum in Conyza Canadensis (0.16 μm). The pollen fertility was found highest in C Canadensis (83.33%) and lowest in Ajuga bracteosa (58.06%). The observed pollen morphology has many valuable qualitative and quantitative attributes for the better understanding of their taxonomy and play significant role in correct identification.

摘要

花粉的微观形态特征已被证明对植物分类学家在植物的鉴定和分类方面有帮助。本研究旨在通过扫描电子显微镜(SEM)和光学显微镜(LM)评估巴基斯坦俾路支省本努地区 Asteraceae 和 Lamiaceae 科的花粉形态特征,以了解其分类学的重要性。从研究区域不同地点采集了七个 Asteraceae 属的四个种和四个 Lamiaceae 属的四个种的花粉。本研究工作提供了详细的花粉形态和花粉壁纹饰特征,包括花粉形状、类型、大小、P/E 比、花粉外壁纹饰和厚度等。在 Asteraceae 和 Lamiaceae 中,花粉类型从三孔到三沟、三孔到三孔到六孔。Ajuga bracteosa 的极轴直径(40.05 μm)和赤道直径(37.66 μm)最大,Isodon rugosus(11.10 μm)和 Erigeron canadensis(13.20 μm)的极轴直径和赤道直径最小。外壁纹饰包括:刺状、网状、细颗粒状、网状皱纹状、网状细颗粒状、穿孔状和网状穿孔状。外壁厚度最大的是 Helianthus tuberosus(1.50 μm),最小的是 Conyza Canadensis(0.16 μm)。花粉育性最高的是 C Canadensis(83.33%),最低的是 Ajuga bracteosa(58.06%)。观察到的花粉形态具有许多有价值的定性和定量属性,有助于更好地理解其分类学,并在正确识别方面发挥重要作用。

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