Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
Microsc Res Tech. 2022 Jan;85(1):373-384. doi: 10.1002/jemt.23912. Epub 2021 Aug 31.
The present study was intended to assess pollen morphological attributes of selected Asteraceous and Brassicaceous species from tehsil Esa Khel (Mianwali), Punjab using scanning electron microscopy (SEM) and light microscopy (LM) techniques for its systematic and taxonomic significance for correct identification. Pollen from 12 different species belongs to two plant families from various distributional localities were collected, acetolyzed and measured. Different palynomorphological features were investigated using LM and SEM techniques. In Asteraceous species, three types of pollen (tricolporate, trizonocolporate, and tetracolporate) were observed. Pollen shape was observed prolate-spheroidal in three species while oblate and oblate-spheroidal were detected in Parthenium hysterophorus and Erigeron bonariensis. While sculpturing pattern of exine were echinate, echinate fenestrate, echinate perforate and scabrate echinate. Mesocolpium measurement was calculated maximum for Sonchus oleraceous (16.6 μm). Brassicaceae pollen were circular, lobate, tricolpate and exine show reticulate peculiarities. Whereas dominant shape was oblate-spheroidal followed by prolate-spheroidal and sub-prolate in Lepidium didymum and Sisymbrium irio, respectively. Mesocolpium distance was noted highest in Raphanus raphanistrum (14.4 μm). Exine thickness was noted maximum in Erigeron bonariensis (2.9 μm) in Asteraceous species and in Brassicaceae; Lepedium didymum exine measurement was 2.7 μm. The study showed that pollen micromorphology has important role to accurately identify and classify diverse plants genera belong to different families. Based on these taxonomic palynomorph features, the accurate identification of species from flora of tehsil Esa Khel, Mianwali were elaborated.
本研究旨在利用扫描电子显微镜（SEM）和光学显微镜（LM）技术评估来自旁遮普埃萨赫尔（密瓦利）的选定 Asteraceae 和 Brassicaceae 物种的花粉形态特征，以了解其对正确识别的系统和分类学意义。从不同分布地点采集了来自两个植物科的 12 种不同物种的花粉，进行乙酰化和测量。使用 LM 和 SEM 技术研究了不同的花粉形态特征。在 Asteraceae 物种中，观察到三种类型的花粉（三孔，三孔和四孔）。在三种物种中观察到花粉形状为长球形，而在 Parthenium hysterophorus 和 Erigeron bonariensis 中则检测到扁球形和扁球形。而外壁纹饰模式为刺状，刺状网纹，刺状穿孔和具刺状。中脊的测量值最大的是 Sonchus oleraceous（16.6 μm）。Brassicaceae 的花粉呈圆形，叶状，三孔，外壁呈网状。而在 Lepidium didymum 和 Sisymbrium irio 中，主要形状为扁球形，其次是长球形和亚长球形。在 Raphanus raphanistrum 中，中脊距离最高（14.4 μm）。在 Asteraceae 物种中，Erigeron bonariensis 的外壁厚度最大（2.9 μm），而在 Brassicaceae 中，Lepedium didymum 的外壁测量值为 2.7 μm。研究表明，花粉微形态在准确识别和分类不同科的不同植物属方面具有重要作用。基于这些分类花粉形态特征，详细阐述了密瓦利埃萨赫尔地区植物区系中物种的准确鉴定。
