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使用16S rRNA广谱PCR测序对血培养阴性标本的微生物学结果：2018年至2022年在加拿大一个省份的回顾性研究

The microbiological outcomes of culture-negative blood specimens using 16S rRNA broad-range PCR sequencing: a retrospective study in a Canadian province from 2018 to 2022.

作者信息

Lieu Anthony, Harrison Luke B, Harel Josée, Lawandi Alexander, Cheng Matthew P, Domingo Marc-Christian

机构信息

McGill University Health Center, Montréal, Québec, Canada.

Laboratoire de santé publique du Québec, Montréal, Québec, Canada.

出版信息

J Clin Microbiol. 2024 Mar 13;62(3):e0151823. doi: 10.1128/jcm.01518-23. Epub 2024 Feb 1.

DOI:10.1128/jcm.01518-23
PMID:38299828
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10935633/
Abstract

UNLABELLED

Broad-range 16S rRNA PCR and sequencing of 1,183 blood specimens from 853 unique patients yielded an interpretable sequence and bacterial identification in 29%, 16S rRNA amplification with uninterpretable sequences in 53%, and no amplification in 18%. This study highlights the potential utility of this technique in identifying fastidious gram-negative and anaerobic bacteria but the frequent recovery of environmental and contaminant organisms argues for its judicious use.

IMPORTANCE

The existing literature focuses on its performance compared to blood cultures in patients with sepsis, leaving a gap in the literature regarding other blood specimens in suspected infectious syndrome across the severity spectrum. We aimed to characterize its microbiological outcomes and provide insight into its potential clinical utility.

摘要

未标注

对来自853名独特患者的1183份血液标本进行广谱16S rRNA聚合酶链反应（PCR）和测序，结果显示29%的标本获得了可解读序列并鉴定出细菌，53%的标本16S rRNA扩增后序列不可解读，18%的标本未扩增。本研究强调了该技术在鉴定苛养革兰氏阴性菌和厌氧菌方面的潜在效用，但频繁检出环境和污染菌表明应谨慎使用该技术。

重要性

现有文献聚焦于该技术在脓毒症患者中与血培养相比的表现，对于疑似感染综合征不同严重程度的其他血液标本，文献存在空白。我们旨在描述其微生物学结果，并深入了解其潜在临床效用。