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季铵化合物诱导豚鼠接触性皮炎:抗原形成机制

Induction of contact dermatitis in guinea pigs by quaternary ammonium compounds: the mechanism of antigen formation.

作者信息

Schallreuter K U, Schulz K H, Wood J M

出版信息

Environ Health Perspect. 1986 Dec;70:229-37. doi: 10.1289/ehp.8670229.

Abstract

Eight quaternary ammonium compounds were tested for their ability to induce contact dermatitis in guinea pigs by using a modified Freund's complete adjuvant test together with the guinea pig maximization test. Only two quaternary ammonium salts of the eight tested could be designated as strong allergens. These two active substances were shown to be capable of stable association with membrane lipids in forming immunogenic complexes. This surface complexation phenomenon was confirmed by using a spin-labeled quaternary ammonium salt which competed for binding sites at the surface of epidermal cells in vivo. Electron spin resonance was used to demonstrate that stable "ion-pairs" are formed between binding sites and the two allergenic preservatives. Furthermore, information was obtained on the kinetics of immunogenic complex formation as well as on the position and orientation of the quaternary ammonium ion at the cell surface.

摘要

通过改良弗氏完全佐剂试验及豚鼠最大化试验,对8种季铵化合物诱发豚鼠接触性皮炎的能力进行了测试。所测试的8种季铵盐中,只有两种可被认定为强变应原。已证明这两种活性物质在形成免疫原性复合物时能够与膜脂稳定结合。使用自旋标记季铵盐证实了这种表面络合现象,该盐在体内可竞争表皮细胞表面的结合位点。利用电子自旋共振证明,结合位点与两种变应原性防腐剂之间形成了稳定的“离子对”。此外,还获得了有关免疫原性复合物形成动力学以及季铵离子在细胞表面的位置和取向的信息。

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本文引用的文献

2
Contact dermatitis from drugs.药物性接触性皮炎
Proc R Soc Med. 1962 Jan;55(1):39-42. doi: 10.1177/003591576205500111.
4
The Langerhans cell.朗格汉斯细胞。
Crit Rev Immunol. 1981 Dec;3(2):95-180.
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Expression of Thy-1 antigen by murine epidermal cells.小鼠表皮细胞中Thy-1抗原的表达。
J Invest Dermatol. 1983 Sep;81(3):282-5. doi: 10.1111/1523-1747.ep12518326.
8
Spin-labeled biomolecules.自旋标记生物分子。
Proc Natl Acad Sci U S A. 1965 Oct;54(4):1010-7. doi: 10.1073/pnas.54.4.1010.

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