Department of Nephrology, Henan Provincial Key Laboratory of Kidney Disease and Immunology, Henan Provincial Clinical Research Center for Kidney Disease, Henan Provincial People's Hospital and People's Hospital of Zhengzhou University, Zhengzhou, China.
Zhengzhou University, Zhengzhou, China.
Kidney Blood Press Res. 2024;49(1):144-154. doi: 10.1159/000535626. Epub 2024 Feb 1.
Accumulating evidence has disclosed that IgA nephropathy (IgAN) could present shortly after the second dose of COVID-19 mRNA vaccine. However, the undying mechanism remains unclear and we aimed to investigate the potential molecular mechanisms.
We downloaded gene expression datasets of COVID-19 mRNA vaccination (GSE201535) and IgAN (GSE104948). Weighted Gene Co-Expression Network Analysis (WGCNA) was performed to identify co-expression modules related to the second dose of COVID-19 mRNA vaccination and IgAN. Differentially expressed genes (DEGs) were screened, and a transcription factor (TF)-miRNA regulatory network and protein-drug interaction were constructed for the shared genes.
WGCNA identified one module associated with the second dose of COVID-19 mRNA vaccine and four modules associated with IgAN. Gene ontology (GO) analyses revealed enrichment of cell cycle-related processes for the COVID-19 mRNA vaccine hub genes and immune effector processes for the IgAN hub genes. We identified 74 DEGs for the second dose of COVID-19 mRNA vaccine and 574 DEGs for IgAN. Intersection analysis with COVID-19 vaccine-related genes led to the identification of two shared genes, TOP2A and CEP55. The TF-miRNA network analysis showed that hsa-miR-144 and ATF1 might regulate the shared hub genes.
This study provides insights into the common pathogenesis of COVID-19 mRNA vaccination and IgAN. The identified pivotal genes may offer new directions for further mechanistic studies of IgAN secondary to COVID-19 mRNA vaccination.
越来越多的证据表明，IgA 肾病（IgAN）可能在接种第二剂 COVID-19 mRNA 疫苗后不久出现。然而，潜在的机制尚不清楚，我们旨在研究潜在的分子机制。
我们下载了 COVID-19 mRNA 疫苗接种（GSE201535）和 IgAN（GSE104948）的基因表达数据集。进行加权基因共表达网络分析（WGCNA）以鉴定与 COVID-19 mRNA 疫苗第二剂接种和 IgAN 相关的共表达模块。筛选差异表达基因（DEGs），并构建转录因子（TF）-miRNA 调控网络和共享基因的蛋白-药物相互作用。
WGCNA 鉴定了一个与 COVID-19 mRNA 疫苗第二剂接种相关的模块和四个与 IgAN 相关的模块。基因本体（GO）分析显示，COVID-19 mRNA 疫苗枢纽基因与细胞周期相关过程富集，IgAN 枢纽基因与免疫效应过程富集。我们确定了 74 个与 COVID-19 mRNA 疫苗第二剂接种相关的 DEG 和 574 个与 IgAN 相关的 DEG。与 COVID-19 疫苗相关基因的交集分析导致鉴定出两个共享基因，TOP2A 和 CEP55。TF-miRNA 网络分析表明，hsa-miR-144 和 ATF1 可能调节共享的枢纽基因。
本研究深入了解了 COVID-19 mRNA 疫苗接种和 IgAN 的共同发病机制。鉴定的关键基因可能为进一步研究 COVID-19 mRNA 疫苗接种引起的 IgAN 的机制提供新的方向。
