Department of Experimental and Clinical Medicine, Università Politecnica delle Marche, Ancona, Italy.
Department of Clinical Sciences, Polytechnic University of Marche, 60126, Ancona, Italy.
Chem Biol Interact. 2024 Feb 25;390:110895. doi: 10.1016/j.cbi.2024.110895. Epub 2024 Feb 1.
Asbestos has been widely used due to its unique characteristics. It is known that exposure to asbestos causes serious damage to health but one species, chrysolite, is still used because it is considered less toxic and not biopersistent in some countries. The aim of our study was to investigate if cellular process underlying the proliferation, differentiation and cell death of placental tissues could be modify in presence of asbestos fibres (50 μg/ml final concentration), long chrysolite fibres (CHR-L) and short chrysolite fibres (CHR-S), using BeWo cell line, an in vitro model that mimics the syncytiotrophoblast (STB), the outer layer of placental villi. Our data demonstrated that none of the fibres analysed alter syncytiotrophoblast formation but all of them induce ROS formation and reduced cell proliferation. Moreover, we showed that only CHR-L fibre induced was able to induce irreversible DNA alterations that carried cells to apoptosis. In fact, BeWo cells exposed to CHR-L fibre showed a significant increase in cleaved CASP3 protein, a marker of apoptosis. These data suggest that CHR-L may induce death of the placental villi leading to impaired placental development. The impairment of placental development is the basis of many gestational pathologies such as preeclampsia and intrauterine growth retardation. Since these pathologies are very dangerous for foetal and maternal life, we suggest to the gynaecologists to carefully evaluate the area of maternal residence, the working environment, the food used, and the materials used daily to avoid contact with these fibres as much as possible.
由于其独特的特性，石棉得到了广泛的应用。众所周知，接触石棉会对健康造成严重损害，但由于在一些国家认为某些种类的石棉（例如温石棉）的毒性较低且不易在体内蓄积，因此仍在使用。我们的研究目的是研究在存在石棉纤维（最终浓度为 50μg/ml）、长温石棉纤维（CHR-L）和短温石棉纤维（CHR-S）的情况下，胎盘组织的增殖、分化和细胞死亡的细胞过程是否会发生改变，使用 BeWo 细胞系，这是一种体外模型，可以模拟胎盘绒毛的合体滋养层（STB）。我们的数据表明，分析的纤维均未改变合胞滋养层的形成，但它们均诱导 ROS 形成和细胞增殖减少。此外，我们表明，只有 CHR-L 纤维才能诱导不可逆的 DNA 改变，从而导致细胞凋亡。实际上，暴露于 CHR-L 纤维的 BeWo 细胞中 cleaved CASP3 蛋白（凋亡的标志物）显著增加。这些数据表明，CHR-L 可能会导致胎盘绒毛死亡，从而导致胎盘发育不良。胎盘发育不良是许多妊娠病理的基础，例如子痫前期和宫内生长受限。由于这些病理对胎儿和产妇的生命非常危险，因此我们建议妇科医生仔细评估产妇居住地、工作环境、所用食物以及日常使用的材料，以尽可能避免接触这些纤维。
