An Ho Jung, Partha Mira A, Lee HoJoon, Lau Billy T, Pavlichin Dmitri S, Almeda Alison, Hooker Anna C, Shin Giwon, Ji Hanlee P
Department of Medical Oncology, St. Vincent's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
Division of Oncology, Department of Medicine, Stanford University School of Medicine, Palo Alto, CA, United States.
Front Oncol. 2024 Jan 18;13:1310054. doi: 10.3389/fonc.2023.1310054. eCollection 2023.
Colon microbiome composition contributes to the pathogenesis of colorectal cancer (CRC) and prognosis. We analyzed 16S rRNA sequencing data from tumor samples of patients with metastatic CRC and determined the clinical implications.
We enrolled 133 patients with metastatic CRC at St. Vincent Hospital in Korea. The V3-V4 regions of the 16S rRNA gene from the tumor DNA were amplified, sequenced on an Illumina MiSeq, and analyzed using the DADA2 package.
After excluding samples that retained <5% of the total reads after merging, 120 samples were analyzed. The median age of patients was 63 years (range, 34-82 years), and 76 patients (63.3%) were male. The primary cancer sites were the right colon (27.5%), left colon (30.8%), and rectum (41.7%). All subjects received 5-fluouracil-based systemic chemotherapy. After removing genera with <1% of the total reads in each patient, 523 genera were identified. Rectal origin, high CEA level (≥10 ng/mL), and presence of lung metastasis showed higher richness. Survival analysis revealed that the presence of ( = 0.052), ( = 0.002), (<0.001), ( = 0.001), ( = 0.007), ( = 0.002), and ( = 0.003) were associated with short overall survival (OS, <24 months), while the presence of was associated with long OS ( = 0.070). From the multivariate analysis, the presence of (hazard ratio [HR], 6.35; 95% confidence interval [CI], 2.38-16.97; <0.001) was associated with poor prognosis along with high CEA level.
Tumor microbiome features may be useful prognostic biomarkers for metastatic CRC.
结肠微生物群组成有助于结直肠癌（CRC）的发病机制和预后。我们分析了转移性CRC患者肿瘤样本的16S rRNA测序数据，并确定了其临床意义。
我们纳入了韩国圣文森特医院的133例转移性CRC患者。从肿瘤DNA中扩增16S rRNA基因的V3-V4区域，在Illumina MiSeq上进行测序，并使用DADA2软件包进行分析。
在排除合并后保留的 reads 总数 <5% 的样本后，对120个样本进行了分析。患者的中位年龄为63岁（范围34-82岁），76例患者（63.3%）为男性。原发癌部位为右半结肠（27.5%）、左半结肠（30.8%）和直肠（41.7%）。所有受试者均接受了基于5-氟尿嘧啶的全身化疗。在去除每个患者中 reads 总数 <1% 的属后，鉴定出523个属。直肠起源、高癌胚抗原（CEA）水平（≥10 ng/mL）和肺转移的存在显示出更高的丰富度。生存分析显示，（=0.052）、（=0.002）、（<0.001）、（=0.001）、（=0.007）、（=0.002）和（=0.003）的存在与总生存期（OS，<24个月）缩短相关，而的存在与长OS相关（=0.070）。多变量分析显示，的存在（风险比[HR]，6.35；95%置信区间[CI]，2.38-16.97；<0.001）与不良预后以及高CEA水平相关。
肿瘤微生物群特征可能是转移性CRC有用的预后生物标志物。
