白花桔梗与丹参通过 MAPK 和 NF-κB 信号通路在 RAW264.7 细胞中的体外免疫增强作用。

In vitro immune-enhancing effects of Platycodon grandiflorum combined with Salvia plebeian via MAPK and NF-κB signaling in RAW264.7 cells.

机构信息

Department of Marine Bio Food Science, Gangneung-Wonju National University, Gangneung, Gangwon, Korea.

Department of Food Science and Technology, Gangneung-Wonju National University, Gangneung, Gangwon, Korea.

出版信息

PLoS One. 2024 Feb 2;19(2):e0297512. doi: 10.1371/journal.pone.0297512. eCollection 2024.

The immune-enhancing activity of the combination of Platycodon grandiflorum and Salvia plebeian extracts (PGSP) was evaluated through macrophage activation using RAW264.7 cells. PGSP (250-1000 μg/mL) showed a higher release of NO in a dose-dependent manner. The results showed that PGSP could significantly stimulate the production of PGE2, COX-2, TNF-α, IL-1β, and IL-6 in RAW264.7 cells and promote iNOS, COX-2, TNF-α, IL-1β, IL-4, and IL-6 mRNA expression. Western blot analysis demonstrated that the protein expression of iNOS and COX-2 and the phosphorylation of ERK, JNK, p38, and NF-κB p65 were greatly increased in PGSP-treated cells. PGSP also promoted the phagocytic activity of RAW264.7 cells. All these results indicated that PGSP might activate macrophages through MAPK and NF-κB signaling pathways. Taken together, PGSP may be considered to have immune-enhancing activity and might be used as a potential immune-enhancing agent.

采用 RAW264.7 细胞评价桔梗和丹参提取物（PGSP）组合的免疫增强活性。PGSP（250-1000μg/ml）以剂量依赖方式表现出更高的 NO 释放。结果表明，PGSP 可显著刺激 RAW264.7 细胞中 PGE2、COX-2、TNF-α、IL-1β 和 IL-6 的产生，并促进 iNOS、COX-2、TNF-α、IL-1β、IL-4 和 IL-6mRNA 表达。Western blot 分析表明，PGSP 处理的细胞中 iNOS 和 COX-2 的蛋白表达以及 ERK、JNK、p38 和 NF-κB p65 的磷酸化水平显著增加。PGSP 还促进了 RAW264.7 细胞的吞噬活性。所有这些结果表明，PGSP 可能通过 MAPK 和 NF-κB 信号通路激活巨噬细胞。综上所述，PGSP 可能具有免疫增强活性，可作为一种潜在的免疫增强剂。