Mohammadi Milad, Norlander Siri, Hedström Martin, Adlercreutz Patrick, Grey Carl
Division of Biotechnology, Department of Chemistry, Lund University, PO Box 124, SE-221 00 Lund, Sweden.
Division of Biotechnology, Department of Chemistry, Lund University, PO Box 124, SE-221 00 Lund, Sweden.
Food Chem. 2024 Jun 1;442:138619. doi: 10.1016/j.foodchem.2024.138619. Epub 2024 Jan 28.
The growth of plant-based food and drink substitutes has led to increased interest in oat-based milk substitute as a dairy milk alternative. Conventional liquid oat base (LOB) production results in a fibre-rich insoluble by-product and loss of valuable macronutrients. This study investigates the use of xylanase enzymes to release insoluble arabinoxylan (AX) fibre and employs different degrees of milling in the LOB manufacturing process, with the aim to reduce insoluble waste and simultaneously increase soluble dietary fibre in oat-based milk substitutes. The combination of decreased mill gap space from 1 to 0.05 mm and addition of GH10 xylanase, resulted in a homogenous LOB product and solubilization of all available AX. Potential prebiotic arabinoxylooligosaccharides of DP3-7 from GH10 hydrolysis were identified using HPAEC-PAD and MS analysis. These findings demonstrate the value of utilizing xylanases and fine-milling in LOB manufacturing, offering a sustainable approach to maximize health benefits of oat-based beverages.
植物性食品和饮料替代品的增长引发了人们对燕麦奶替代品作为牛奶替代品的兴趣增加。传统的液态燕麦基料（LOB）生产会产生富含纤维的不溶性副产品，并导致宝贵的大量营养素流失。本研究调查了使用木聚糖酶释放不溶性阿拉伯木聚糖（AX）纤维，并在LOB制造过程中采用不同程度的研磨，目的是减少不溶性废物，同时增加燕麦奶替代品中的可溶性膳食纤维。将研磨间隙从1毫米减小到0.05毫米并添加GH10木聚糖酶，得到了均匀的LOB产品，并使所有可用的AX溶解。使用HPAEC-PAD和MS分析鉴定了来自GH10水解的潜在益生元阿拉伯木寡糖DP3-7。这些发现证明了在LOB制造中使用木聚糖酶和精细研磨的价值，为最大化燕麦基饮料的健康益处提供了一种可持续的方法。
