固定时间对大鼠胚胎中2'-脱氧核糖核苷5'-三磷酸池测量的影响。
Effect of fixation time on measurement of 2'-deoxyribonucleoside 5'-triphosphate pools in the rat embyro.
作者信息
Ritter E J, Scott W J, Bruce L M
出版信息
Biochem J. 1979 Jun 1;179(3):715-7. doi: 10.1042/bj1790715.
Abstract
Values for the concentrations of deoxyribonucleoside triphosphates in rat embryos on day 12 of gestation, determined by high-pressure liquid chromatography, were artifactually two to three times as high in embryos fixed by cooling in ice/water followed by freezing on solid CO2, in 20s, as in those more rapidly/fixed in liquid N2, in 1 s.
摘要
通过高压液相色谱法测定，妊娠第12天大鼠胚胎中脱氧核糖核苷三磷酸的浓度值显示，先在冰/水中冷却20秒，然后在固态二氧化碳上冷冻固定的胚胎，其测定值比在液氮中1秒内快速固定的胚胎人为地高出两到三倍。
相似文献
1
Effect of fixation time on measurement of 2'-deoxyribonucleoside 5'-triphosphate pools in the rat embyro.固定时间对大鼠胚胎中2'-脱氧核糖核苷5'-三磷酸池测量的影响。
Biochem J. 1979 Jun 1;179(3):715-7. doi: 10.1042/bj1790715.
2
Effect of 5-fluoro-2'-deoxyuridine on deoxyribonucleotide pools in vivo.5-氟-2'-脱氧尿苷对体内脱氧核苷酸库的影响。
J Natl Cancer Inst. 1980 Sep;65(3):603-5.
3
Changes in ribo- and deoxyribonucleoside triphosphate pools within the cell cycle of a synchronized mouse fibroblast cell line.
Biochim Biophys Acta. 1983 Mar 15;756(1):36-40. doi: 10.1016/0304-4165(83)90021-1.
4
5
Sample preparation and high-performance liquid chromatographic analysis of deoxyribonucleoside triphosphates in individual rat embryos.单个大鼠胚胎中脱氧核糖核苷三磷酸的样品制备及高效液相色谱分析
Anal Biochem. 1998 Jun 1;259(2):245-52. doi: 10.1006/abio.1998.2647.
6
Recovery from vitamin B-12-induced unbalanced growth. The shortened cell cycle and the deoxyribonucleoside triphosphate pools.维生素B-12诱导的生长失衡的恢复。缩短的细胞周期和脱氧核糖核苷三磷酸池。
Biochem J. 1978 Mar 15;170(3):631-6. doi: 10.1042/bj1700631.
7
The quantitative determination of deoxyribonucleoside triphosphates using high performance liquid chromatogrphy.
Biochem Med. 1979 Feb;21(1):16-21. doi: 10.1016/0006-2944(79)90050-4.
8
9
Deoxyribonucleoside triphosphate pools in herpes simplex type 1 infected cells.1型单纯疱疹病毒感染细胞中的脱氧核糖核苷三磷酸库
J Gen Virol. 1976 Apr;31(1):101-13. doi: 10.1099/0022-1317-31-1-101.
10
The molecular basis of mutations induced by deoxyribonucleoside triphosphate pool imbalances in mammalian cells.
Exp Cell Res. 1989 Apr;181(2):305-16. doi: 10.1016/0014-4827(89)90090-6.
本文引用的文献
1
Free nucleotides of the brain in various mammals.不同哺乳动物大脑中的游离核苷酸。
J Neurochem. 1961 Nov;8:116-25. doi: 10.1111/j.1471-4159.1961.tb13533.x.
2
[A simple technic for extremely rapid freezing of large pieces of tissue].[一种用于极快速冷冻大块组织的简单技术]
Pflugers Arch Gesamte Physiol Menschen Tiere. 1960;270:399-412.
3
Nucleotide pools and [6-14C]orotic acid incorporation in early regenerating rat liver.早期再生大鼠肝脏中的核苷酸池及[6-¹⁴C]乳清酸掺入
Biochim Biophys Acta. 1966 Dec 21;129(3):445-59. doi: 10.1016/0005-2787(66)90060-8.
4
Teratogenesis and inhibition of DNA synthesis induced in rat embryos by cytosine arabinoside.
Teratology. 1971 Feb;4(1):7-13. doi: 10.1002/tera.1420040103.
5
Free nucleotides in animal tissues.动物组织中的游离核苷酸。
Prog Nucleic Acid Res Mol Biol. 1964;3:299-334. doi: 10.1016/s0079-6603(08)60744-8.
6
The use of microwave radiation in the determination of acetylcholine in the rat brain.
Brain Res. 1972 Mar 24;38(2):377-89. doi: 10.1016/0006-8993(72)90720-2.
7
The ATP pool in Escherichia coli. I. Measurement of the pool using modified luciferase assay.大肠杆菌中的ATP库。I. 使用改良的荧光素酶测定法测量ATP库
Biochim Biophys Acta. 1967;143(3):445-53. doi: 10.1016/0005-2728(67)90050-3.
8
Cell-cycle-dependent variations of deoxyribonucleoside triphosphate pools in Chinese hamster cells.中国仓鼠细胞中脱氧核糖核苷三磷酸库的细胞周期依赖性变化。
Biochim Biophys Acta. 1973 Sep 7;319(3):336-47. doi: 10.1016/0005-2787(73)90173-1.
9
Deoxyribonucleotide pools during liver regeneration.肝脏再生过程中的脱氧核苷酸池。
Eur J Biochem. 1973 Feb 15;33(1):36-9. doi: 10.1111/j.1432-1033.1973.tb02651.x.
10
Analysis of nucleotide pools in animal cells.
Methods Cell Biol. 1973;7:361-462. doi: 10.1016/s0091-679x(08)61787-2.
Zotero 插件