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固定时间对大鼠胚胎中2'-脱氧核糖核苷5'-三磷酸池测量的影响。

Effect of fixation time on measurement of 2'-deoxyribonucleoside 5'-triphosphate pools in the rat embyro.

作者信息

Ritter E J, Scott W J, Bruce L M

出版信息

Biochem J. 1979 Jun 1;179(3):715-7. doi: 10.1042/bj1790715.

DOI:10.1042/bj1790715
PMID:383073
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1186684/
Abstract

Values for the concentrations of deoxyribonucleoside triphosphates in rat embryos on day 12 of gestation, determined by high-pressure liquid chromatography, were artifactually two to three times as high in embryos fixed by cooling in ice/water followed by freezing on solid CO2, in 20s, as in those more rapidly/fixed in liquid N2, in 1 s.

摘要

通过高压液相色谱法测定，妊娠第12天大鼠胚胎中脱氧核糖核苷三磷酸的浓度值显示，先在冰/水中冷却20秒，然后在固态二氧化碳上冷冻固定的胚胎，其测定值比在液氮中1秒内快速固定的胚胎人为地高出两到三倍。

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本文引用的文献

1
Free nucleotides of the brain in various mammals.不同哺乳动物大脑中的游离核苷酸。
J Neurochem. 1961 Nov;8:116-25. doi: 10.1111/j.1471-4159.1961.tb13533.x.
2
[A simple technic for extremely rapid freezing of large pieces of tissue].[一种用于极快速冷冻大块组织的简单技术]
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Nucleotide pools and [6-14C]orotic acid incorporation in early regenerating rat liver.早期再生大鼠肝脏中的核苷酸池及[6-¹⁴C]乳清酸掺入
Biochim Biophys Acta. 1966 Dec 21;129(3):445-59. doi: 10.1016/0005-2787(66)90060-8.
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Teratogenesis and inhibition of DNA synthesis induced in rat embryos by cytosine arabinoside.
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Free nucleotides in animal tissues.动物组织中的游离核苷酸。
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The use of microwave radiation in the determination of acetylcholine in the rat brain.
Brain Res. 1972 Mar 24;38(2):377-89. doi: 10.1016/0006-8993(72)90720-2.
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The ATP pool in Escherichia coli. I. Measurement of the pool using modified luciferase assay.大肠杆菌中的ATP库。I. 使用改良的荧光素酶测定法测量ATP库
Biochim Biophys Acta. 1967;143(3):445-53. doi: 10.1016/0005-2728(67)90050-3.
8
Cell-cycle-dependent variations of deoxyribonucleoside triphosphate pools in Chinese hamster cells.中国仓鼠细胞中脱氧核糖核苷三磷酸库的细胞周期依赖性变化。
Biochim Biophys Acta. 1973 Sep 7;319(3):336-47. doi: 10.1016/0005-2787(73)90173-1.
9
Deoxyribonucleotide pools during liver regeneration.肝脏再生过程中的脱氧核苷酸池。
Eur J Biochem. 1973 Feb 15;33(1):36-9. doi: 10.1111/j.1432-1033.1973.tb02651.x.
10
Analysis of nucleotide pools in animal cells.
Methods Cell Biol. 1973;7:361-462. doi: 10.1016/s0091-679x(08)61787-2.