Jamieson A T, Bjursell G
J Gen Virol. 1976 Apr;31(1):101-13. doi: 10.1099/0022-1317-31-1-101.
Deoxyribonucleoside triphosphate pools were analysed in both exponentially growing and serum starved wild type BHK C13 cells and in a derivative of this cell line which lacks both thymidine kinase and deoxycytidine kinase activities, before and after infection with herpes simplex virus. Serum starved BHK cells had low levels of all four deoxyribonucleoside triphosphates. In exponentially growing cells all pools were expanded, the pool of dCTP being largest and dGTP the smallest. The dATP and dTTP pools were of intermediate sizes. In exponentially growing deoxypyrimidine kinase free cells the pools, with respect to level and distribution, were the same as those observed in wild type cells. After infection with herpes simplex virus there were marked changes in the levels of all deoxyribonucleoside triphosphate pools; the most predominant being a 25- to 50-fold expansion of dTTP pool. The pools of dCTP and dGTP also increased while the pool of dATP was very much reduced. These effects could be observed in both wild type and mutant cells.
在单纯疱疹病毒感染之前和之后，对指数生长和血清饥饿的野生型BHK C13细胞以及该细胞系的一个衍生物（缺乏胸苷激酶和脱氧胞苷激酶活性）中的脱氧核糖核苷三磷酸库进行了分析。血清饥饿的BHK细胞中所有四种脱氧核糖核苷三磷酸的水平都很低。在指数生长的细胞中，所有的库都扩大了，dCTP库最大，dGTP库最小。dATP和dTTP库的大小处于中间水平。在指数生长的无脱氧嘧啶激酶细胞中，库的水平和分布与野生型细胞中观察到的相同。感染单纯疱疹病毒后，所有脱氧核糖核苷三磷酸库的水平都有明显变化；最主要的是dTTP库扩大了25至50倍。dCTP和dGTP库也增加了，而dATP库则大大减少。这些效应在野生型和突变型细胞中都能观察到。
