Department of Anatomy, Faculty of Medicine, Universiti Teknologi MARA, Jalan Hospital 47000, Sungai Buloh, Selangor, Malaysia; Institute for Pathology, Laboratory and Forensic Medicine (I-PPerForM), Universiti Teknologi MARA, Jalan Hospital, 47000, Sungai Buloh, Selangor, Malaysia.
Institute for Pathology, Laboratory and Forensic Medicine (I-PPerForM), Universiti Teknologi MARA, Jalan Hospital, 47000, Sungai Buloh, Selangor, Malaysia; Department of Pathology, Faculty of Medicine, Universiti Teknologi MARA, Jalan Hospital, 47000, Sungai Buloh, Selangor, Malaysia.
J Forensic Leg Med. 2024 Feb;102:102654. doi: 10.1016/j.jflm.2024.102654. Epub 2024 Feb 1.
Coronary atherosclerosis is due to build-up of plaque within the coronary arteries. Post-mortem computed tomography (PMCT) allows non or minimally invasive visualization of abnormalities prior to an autopsy, however PMCT-angiography (PMCTA) greatly enhances relevant findings, especially in viewing the cardiovascular system which is important in the diagnosis of coronary atherosclerosis. Contrast media used in PMCTA however has been reported to cause distortion of tissue which may interfere with post-mortem investigation outcomes. A cross sectional study to investigate the effect of PMCTA on tissue biomarkers in coronary arteries was performed involving cases brought in dead to the Institute and Accident and Emergency Unit. Sixty-three autopsy cases were included in this study, whereby 18 cases underwent PMCT while 45 cases underwent PMCTA. The subjects subsequently had a conventional autopsy where coronary artery sections were collected for standard histological examination and immunohistochemistry examination for endothelial inflammatory (CD36), prothrombogenic (TPA) and plaque stability (MMP-9) markers. The subjects consisted of 55 males and 8 females with a mean age ±SD of 49 ± 18.11 years. There were no significant differences in the coronary artery endothelial expression of CD36, MMP-9 and TPA between PMCT and PMCTA subjects. PMCTA does not alter CD36, TPA and MMP-9 markers supporting the safe use of PMCTA in post-mortem examinations.
冠状动脉粥样硬化是由于冠状动脉内斑块的形成。死后计算机断层扫描（PMCT）允许在尸检前对异常情况进行非侵入性或微创可视化，然而 PMCT 血管造影（PMCTA）极大地增强了相关发现，特别是在观察心血管系统方面，这对冠状动脉粥样硬化的诊断很重要。然而，PMCTA 中使用的对比剂已被报道会导致组织变形，这可能会干扰死后调查结果。进行了一项横断面研究，以调查 PMCTA 对冠状动脉组织生物标志物的影响，该研究涉及送到研究所和急症室的死亡病例。本研究共纳入 63 例尸检病例，其中 18 例接受了 PMCT，45 例接受了 PMCTA。随后，对这些病例进行了常规尸检，收集冠状动脉切片进行标准组织学检查和免疫组织化学检查，以检测内皮炎症（CD36）、促血栓形成（TPA）和斑块稳定性（MMP-9）标志物。这些病例包括 55 名男性和 8 名女性，平均年龄 ±标准差为 49 ± 18.11 岁。PMCT 和 PMCTA 组的冠状动脉内皮 CD36、MMP-9 和 TPA 表达无显著差异。PMCTA 不会改变 CD36、TPA 和 MMP-9 标志物，支持在死后检查中安全使用 PMCTA。
