Gilani Sadaf Jamal, Bin Jumah May Nasser, Fatima Farhat, Al-Abbasi Fahad A, Afzal Muhammad, Alzarea Sami I, Sayyed Nadeem, Nadeem Muhammad Shahid, Kazmi Imran
Department of Basic Health Sciences, Foundation Year, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia.
Environment and Biomaterial Unit, Health Sciences Research Center, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia.
PeerJ. 2024 Jan 31;12:e16795. doi: 10.7717/peerj.16795. eCollection 2024.
This study explores the neuroprotective potential of hibiscetin concerning memory deficits induced by lipopolysaccharide (LPS) injection in rats. The aim of this study is to evaluate the effect of hibiscetin against LPS-injected memory deficits in rats. The behavioral paradigms were conducted to access LPS-induced memory deficits. Various biochemical parameters such as acetyl-cholinesterase activity, choline-acetyltransferase, antioxidant (superoxide dismutase, glutathione transferase, catalase), oxidative stress (malonaldehyde), and nitric oxide levels were examined. Furthermore, neuroinflammatory parameters such as tumor necrosis factor-α, interleukin-1β (IL-1β), IL-6, and nuclear factor-kappa B expression and brain-derived neurotrophic factor as well as apoptosis marker ., caspase-3 were evaluated. The results demonstrated that the hibiscetin-treated group exhibited significant recovery in LPS-induced memory deficits in rats by using behavioral paradigms, biochemical parameters, antioxidant levels, oxidative stress, neuroinflammatory markers, and apoptosis markers. Recent research suggested that hibiscetin may serve as a promising neuroprotective agent in experimental animals and could offer an alternative in LPS-injected memory deficits in rodent models.
本研究探讨了木槿黄酮对脂多糖（LPS）注射诱导的大鼠记忆缺陷的神经保护潜力。本研究的目的是评估木槿黄酮对LPS注射诱导的大鼠记忆缺陷的影响。通过行为范式来评估LPS诱导的记忆缺陷。检测了各种生化参数，如乙酰胆碱酯酶活性、胆碱乙酰转移酶、抗氧化剂（超氧化物歧化酶、谷胱甘肽转移酶、过氧化氢酶）、氧化应激（丙二醛）和一氧化氮水平。此外，还评估了神经炎症参数，如肿瘤坏死因子-α、白细胞介素-1β（IL-1β）、IL-6和核因子-κB表达以及脑源性神经营养因子和凋亡标志物，即半胱天冬酶-3。结果表明，通过行为范式、生化参数、抗氧化剂水平、氧化应激、神经炎症标志物和凋亡标志物评估，木槿黄酮治疗组的大鼠在LPS诱导的记忆缺陷方面表现出显著恢复。最近的研究表明，木槿黄酮可能是实验动物中有前景的神经保护剂，并且可以为啮齿动物模型中LPS注射引起的记忆缺陷提供一种替代方法。
