Rohit Singh T, Ezhilarasan Devaraj, Karthick Munusamy, Shree Harini Karthik
Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IND.
Cureus. 2024 Jan 3;16(1):e51609. doi: 10.7759/cureus.51609. eCollection 2024 Jan.
Background Drug-induced liver injury is a common cause of acute liver failure. Isoniazid (INH) is used as a first-line treatment for tuberculosis. Clinical and experimental studies have reported abnormal liver function after INH therapy. Pers., commonly known as banaba, has been traditionally used to treat various ailments including diabetes and obesity due to its antioxidant and anti-inflammatory properties. Aim To investigate the hepatoprotective effect of ethanolic banaba leaf extract (EBLE) against INH-induced hepatotoxicity in rats. Materials and methods A total of 30 male Wistar albino rats (150 - 200 g) were divided into five groups (n = 6). Group I rats were served as a control and were administered dimethyl sulfoxide for the first 30 days and water for the next 30 consecutive days. Group II rats were administered INH (50 mg/kg, p.o.) once in the first 30 consecutive days and sacrificed at Day 30. Group III rats were administered INH for 30 consecutive days and left without treatment for the next 30 days. In Groups IV and V, rats were post-treated orally with EBLE 250 and 500 mg/kg, p.o. (0.3 ml/rat) for 30 days after INH administration. At the end of Day 60, the remaining group of animals were sacrificed. The blood and liver tissues were collected. The marker enzymes of hepatotoxicity, oxidative stress markers, inflammatory markers, and histopathology were analyzed. Results INH administration induced significant elevation of marker enzymes (aspartate transaminase, alanine transaminase, alkaline phosphatase, lactate dehydrogenase, bilirubin, gamma-glutamyl transpeptidase) of hepatotoxicity in the serum. This treatment also increased lipid peroxidation and proinflammatory marker expression (tumor necrosis factor-alpha, transforming growth factor-beta, and nuclear factor kappa B (NF-κB) except inhibitor of NF-κB) and decreased antioxidants such superoxide dismutase, catalase, and glutathione in the liver tissue. All these abnormalities were significantly mitigated after treatment with EBLE. Conclusion The results of this study suggest that EBLE can be used for INH-induced hepatotoxicity.
背景 药物性肝损伤是急性肝衰竭的常见原因。异烟肼（INH）用作结核病的一线治疗药物。临床和实验研究报告了INH治疗后肝功能异常。匙羹藤，俗称巴拿巴，因其抗氧化和抗炎特性，传统上用于治疗包括糖尿病和肥胖症在内的各种疾病。目的 研究匙羹藤叶乙醇提取物（EBLE）对INH诱导的大鼠肝毒性的肝保护作用。材料和方法 总共30只雄性Wistar白化大鼠（150 - 200克）分为五组（n = 6）。第一组大鼠作为对照，在最初30天给予二甲基亚砜，随后连续30天给予水。第二组大鼠在连续的前30天内每天口服给予INH（50毫克/千克），并在第30天处死。第三组大鼠连续30天给予INH，然后在接下来的30天不进行治疗。在第四组和第五组中，大鼠在给予INH后口服EBLE 250和500毫克/千克（0.3毫升/只大鼠），持续30天。在第60天结束时，处死其余动物组。收集血液和肝脏组织。分析肝毒性标志物酶、氧化应激标志物、炎症标志物和组织病理学。结果 给予INH导致血清中肝毒性标志物酶（天冬氨酸转氨酶、丙氨酸转氨酶、碱性磷酸酶、乳酸脱氢酶、胆红素、γ-谷氨酰转肽酶）显著升高。这种治疗还增加了脂质过氧化和促炎标志物表达（肿瘤坏死因子-α、转化生长因子-β和核因子κB（NF-κB），除了NF-κB抑制剂），并降低了肝脏组织中的抗氧化剂如超氧化物歧化酶、过氧化氢酶和谷胱甘肽。用EBLE治疗后，所有这些异常均得到显著缓解。结论 本研究结果表明，EBLE可用于治疗INH诱导的肝毒性。
