Somayaji Ranjani, Thornton Christina S, Acosta Nicola, Smith Kristine, Clark Jessica, Fatovich Linda, Thakrar Mitesh V, Parkins Michael D
Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine University of Calgary Calgary Canada.
Department of Medicine, Cumming School of Medicine, Alberta Health Services University of Calgary Calgary Canada.
OTO Open. 2024 Feb 5;8(1):e101. doi: 10.1002/oto2.101. eCollection 2024 Jan-Mar.
Sinus disease is prevalent in persons with cystic fibrosis (PwCF) and may be a reservoir of airway infection in postlung transplant (pTx) patients. The microbial composition of cystic fibrosis sinuses and its associations with chronic rhinosinusitis (CRS) is relatively unexplored. We aimed to examine the sinus and lower airway microbiome and their associations with CRS in PwCF and pTxPwCF.
Prospective single-centre study.
A total of 31 sex and age (±2 years) matched PwCF and pTxPwCF.
Demographic and clinical data along with sinus swabs and sputum were collected. CRS was assessed using Sinonasal Outcome Test-22 (SNOT-22) (patient reported outcome) and Lund-McKay (computed tomography sinus) scores. Samples underwent MiSeq Illumina sequencing of the universal 16S ribosomal RNA gene.
A total of 31 PwCF (15 pTxPwCF) were included. Aggregate airways microbiome composition was dominated by (46%), (14%), (11%), (10%), and (6%). α-diversity was significantly lower in post-Tx samples across both sputum and sinus samples ( = .005). β-diversity was significantly different between sputum ( = .004), but not sinus ( = .75) samples by transplant status. While there was a trend in higher β-diversity associated with lower SNOT-22 score at time of first visit, this did not reach significance ( = .05).
Sinus and airway microbiomes differed in PwCF and pTxPwCF, but the prevalent organisms remained consistent. Elucidating the relationship of the microbiome with clinical status to better understand when to intervene accordingly is needed to optimize sinus disease management in PwCF.
鼻窦疾病在囊性纤维化患者（PwCF）中很常见，并且可能是肺移植后（pTx）患者气道感染的一个病灶。囊性纤维化鼻窦的微生物组成及其与慢性鼻-鼻窦炎（CRS）的关联相对未被充分研究。我们旨在研究PwCF和pTxPwCF患者的鼻窦和下呼吸道微生物群及其与CRS的关联。
前瞻性单中心研究。
共31名年龄和性别匹配（±2岁）的PwCF和pTxPwCF患者。
收集人口统计学和临床数据以及鼻窦拭子和痰液。使用鼻鼻窦结局测试-22（SNOT-22）（患者报告结局）和伦德-麦凯（鼻窦计算机断层扫描）评分评估CRS。样本进行通用16S核糖体RNA基因的MiSeq Illumina测序。
共纳入31名PwCF患者（15名pTxPwCF患者）。气道微生物群的总体组成以[具体微生物1]（46%）、[具体微生物2]（14%）、[具体微生物3]（11%）、[具体微生物4]（10%）和[具体微生物5]（6%）为主。移植后样本中痰液和鼻窦样本的α多样性均显著降低（P = 0.005）。按移植状态分类，痰液样本的β多样性有显著差异（P = 0.004），但鼻窦样本无显著差异（P = 0.75）。虽然首次就诊时β多样性较高与较低的SNOT-22评分存在一定趋势，但未达到显著水平（P = 0.05）。
PwCF和pTxPwCF患者的鼻窦和气道微生物群存在差异，但优势微生物保持一致。需要阐明微生物群与临床状态的关系，以便更好地了解何时进行相应干预，从而优化PwCF患者鼻窦疾病的管理。
