Theriogenology Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Pharmacology Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
J Anim Physiol Anim Nutr (Berl). 2024 May;108(3):839-853. doi: 10.1111/jpn.13933. Epub 2024 Feb 7.
This experiment was designed to investigate the impact of curcumin-olive oil nanocomposite (CONC) supplementation on uteroplacental hemodynamics and ultrasonographic measurements as well as maternal oxidative status in midgestating goats. Twelve synchronized pregnant goats (85.58 ± 1.08 days of gestation; mean ± SD) were uniformly assigned to two groups (n = 6/group); the first group received daily oral supplementation of CONC (3 mg/kg body weight; nanocurcumin [NC] group) for 32 days, and the second group was offered physiological saline (control) following the NC group timeline. The goats of both groups were examined at 3-day intervals for middle uterine (MUA) and umbilical (UMA) arteries hemodynamics (pulsatility index [PI], resistive index [RI], systole/diastole [S/D] and blood flow rate [BFR]) and diameters, uteroplacental thickness (UPT), placentomes' diameter (PD) and echogenicity, steroid hormones (progesterone and estradiol 17β), oxidative biomarkers (total antioxidant capacity [TAC], catalase [CAT], malondialdehyde [MDA]), nitric oxide (NO) and blood cells DNA integrity. The UPT (p = 0.012) and PD (p = 0.021) values were higher in the NC group than in their counterparts' control group (D11-32). There were increases in diameter (p = 0.021 and p = 0.012) and decreases (p = 0.021, p = 0.016 and p = 0.041 [MUA]; p = 0.015, p = 0.023 and p = 0.011 [UMA] respectively) in Doppler indices (PI, RI and S/D) of the MUA and UMA in the NC group compared to the control group (D14-32). On D20-32 (MUA) and D14-32 (UMA), the NC goats had higher BFR than the control group (p = 0.021, 0.018 respectively). The means of blood cells with fragmented DNA were lower (p = 0.022) in the NC group than in the control group on Days 8 and 21 postsupplementation. There were increases in CAT and NO (D20-32; p = 0.022 and p = 0.004 respectively), and TAC (D17-32; p = 0.007) levels in the NC goats compared to the control ones. The NC group had lower (p = 0.029) concentrations of MDA than the control group on Day 20 postsupplementation onward. In conclusion, oral supplementation of CONC improved uteroplacental blood flow and the antioxidant capacity of midgestating goats.
本实验旨在研究姜黄素-橄榄油纳米复合物（CONC）补充对中期妊娠山羊子宫胎盘血液动力学和超声测量以及母体氧化状态的影响。12 只同步妊娠山羊（妊娠 85.58±1.08 天；均值±标准差）均匀分为两组（n=6/组）；第一组每天口服 CONC（3mg/kg 体重；纳米姜黄素[NC]组）32 天，第二组在 NC 组之后给予生理盐水（对照）。两组山羊每隔 3 天检查一次子宫中动脉（MUA）和脐动脉（UMA）的血液动力学（搏动指数[PI]、阻力指数[RI]、收缩期/舒张期[S/D]和血流速度[BFR]）和直径、子宫胎盘厚度（UPT）、胎盘直径（PD）和回声、甾体激素（孕酮和雌二醇 17β）、氧化生物标志物（总抗氧化能力[TAC]、过氧化氢酶[CAT]、丙二醛[MDA]）、一氧化氮（NO）和血细胞 DNA 完整性。与对照组相比，NC 组的 UPT（p=0.012）和 PD（p=0.021）值更高（D11-32）。NC 组的直径增加（p=0.021 和 p=0.012）和多普勒指数（PI、RI 和 S/D）降低（p=0.021、p=0.016 和 p=0.041[MUA]；p=0.015、p=0.023 和 p=0.011[UMA]）（D14-32）与对照组相比，MUA 和 UMA 的分别。在 D20-32（MUA）和 D14-32（UMA），NC 组的 BFR 高于对照组（p=0.021，0.018 分别）。补充后第 8 天和第 21 天，NC 组血细胞 DNA 碎片化程度低于对照组（p=0.022）。与对照组相比，NC 组 CAT 和 NO（D20-32；p=0.022 和 p=0.004）水平升高，TAC（D17-32；p=0.007）水平升高。与对照组相比，NC 组在补充后第 20 天开始 MDA 浓度较低（p=0.029）。总之，口服 CONC 补充可改善中期妊娠山羊的子宫胎盘血流和抗氧化能力。
