Miller S E, Finnerty W R
Can J Microbiol. 1979 Jun;25(6):765-72. doi: 10.1139/m79-111.
From electron-microscopical observations, a decreased metabolic activity in 3-day-old Candida albicans chlamydospores was suggested, and progressive deterioration in chlamydospores aged 2-8 months was shown. Oxygen utilization by chlamydospore-pseudomycelium (CSP) preparations was less than that by yeast, while 3-day-old CSP preparations used significantly less O2 than 24-h CSP preparations. Amino acid incorporation was greater in yeast than in CSP preparations. Leucine incorporation by 20-h yeasts was twice that of 5-day yeasts and 5 times that of 20-h and 5-day CSP. Amino acid decarboxylation was similar in yeasts and CSP and was determined by end-product analyses to be via amino acid oxidase. Light microscopy autoradiography of [14C]leucine incorporation demonstrated that the metabolic activity in CSP preparations was due to the young growing tips of the pseudomycelium and not to mature chlamydospores. Yeasts did not take up trypan blue could be stained if first autoclaved or treated with 10% acid or 10% base. Young chlamydospores grown in the presence of trypan blue developed unstained and became permeable to the dye at 2 1/2 days. These data suggest that chlamydospores of C. albicans do not function in the classical role attributed to spores; i.e., mature chlamydospores cannot germinate, but rather age, deteriorate, and die.
电子显微镜观察表明，3日龄白色念珠菌厚垣孢子的代谢活性降低，且显示出2 - 8个月龄厚垣孢子的渐进性退化。厚垣孢子-假菌丝（CSP）制剂的氧气利用率低于酵母，而3日龄CSP制剂的耗氧量明显低于24小时龄的CSP制剂。酵母中的氨基酸掺入量高于CSP制剂。20小时龄酵母的亮氨酸掺入量是5日龄酵母的两倍，是20小时龄和5日龄CSP的5倍。酵母和CSP中的氨基酸脱羧作用相似，通过终产物分析确定是通过氨基酸氧化酶进行的。[14C]亮氨酸掺入的光学显微镜放射自显影表明，CSP制剂中的代谢活性归因于假菌丝的年轻生长尖端，而非成熟的厚垣孢子。酵母不摄取台盼蓝，但若先进行高压灭菌或用10%酸或10%碱处理则可被染色。在台盼蓝存在下生长的年轻厚垣孢子在2.5天时未被染色，且对染料变得通透。这些数据表明，白色念珠菌的厚垣孢子并不发挥孢子的经典作用；即成熟的厚垣孢子不能萌发，而是老化、退化并死亡。
