Department of Animal Science, Jeonbuk National University, Jeonju 54896, Republic of Korea.
Division of Animal Science, Chonnam National University, Gwang-Ju 61186, Republic of Korea.
J Microbiol Biotechnol. 2024 Apr 28;34(4):854-862. doi: 10.4014/jmb.2312.12035. Epub 2024 Jan 29.
is a commonly used probiotic, and many researchers have focused on its stress response to improve its functionality and survival. However, studies on persister cells, dormant cells that aid bacteria in surviving general stress, have focused on pathogenic bacteria that cause infection, not . Thus, understanding persister cells will provide essential clues for understanding how survives and maintains its function under various environmental conditions. We treated strains with various antibiotics to determine the conditions required for persister formation using kill curves and transmission electron microscopy. In addition, we observed the resuscitation patterns of persister cells using single-cell analysis. Our results show that creates a small population of persister cells (0.0001-1% of the bacterial population) in response to beta-lactam antibiotics such as ampicillin and amoxicillin. Moreover, only around 0.5-1% of persister cells are heterogeneously resuscitated by adding fresh media; the characteristics are typical of persister cells. This study provides a method for forming and verifying the persistence of and demonstrates that antibiotic-induced persister cells show characteristics of dormancy, sensitivity of antibiotics, same as exponential cells, multi-drug tolerance, and resuscitation, which are characteristics of general persister cells. This study suggests that the mechanisms of formation and resuscitation may vary depending on the characteristics, such as the membrane structure of the bacterial species.
是一种常用的益生菌，许多研究人员都专注于其应激反应，以提高其功能和生存能力。然而，关于休眠细胞（帮助细菌在一般压力下存活的休眠细胞）的研究集中在引起感染的病原菌上，而不是 。因此，了解 休眠细胞将为理解 如何在各种环境条件下生存和维持其功能提供重要线索。我们用各种抗生素处理 菌株，使用致死曲线和透射电子显微镜来确定形成休眠细胞的条件。此外，我们还通过单细胞分析观察了休眠细胞的复苏模式。我们的结果表明， 对氨苄西林和阿莫西林等β-内酰胺类抗生素会产生一小部分休眠细胞（占细菌群体的 0.0001-1%）。此外，只有约 0.5-1%的休眠细胞通过添加新鲜培养基异质复苏；这些特征是休眠细胞的典型特征。本研究提供了一种形成和验证 休眠的方法，并表明抗生素诱导的 休眠细胞表现出休眠、抗生素敏感性、与指数细胞相同、多药耐药性和复苏的特征，这些都是一般休眠细胞的特征。本研究表明，形成和复苏的机制可能因细菌种类的膜结构等特征而有所不同。
