College of Biosciences and Biotechnology, Shenyang Agricultural University, Shenyang 110065, China.
School of Life Sciences, Guangzhou University, Guangdong 511370, China.
ACS Synth Biol. 2024 Feb 16;13(2):428-448. doi: 10.1021/acssynbio.3c00685. Epub 2024 Feb 7.
The CRISPR/Cas9 systems have been developed as tools for genetic engineering and metabolic engineering in various organisms. In this review, various aspects of CRISPR/Cas9 in , from basic principles to practical applications, have been summarized. First, a comprehensive review has been conducted on the history of CRISPR/Cas9, successful cases of gene disruptions, and efficiencies of multiple DNA fragment insertions. Such advanced systems have accelerated the development of microbial engineering by reducing time and labor, and have enhanced the understanding of molecular genetics. Furthermore, the research progress of the CRISPR/Cas9-based systems in the production of high-value-added chemicals and the improvement of stress tolerance in have been summarized, which should have an important reference value for genetic and synthetic biology studies based on .
CRISPR/Cas9 系统已被开发为各种生物的遗传工程和代谢工程的工具。在这篇综述中，从基本原理到实际应用，总结了 CRISPR/Cas9 在 中的各个方面。首先，对 CRISPR/Cas9 的历史、基因敲除的成功案例和多个 DNA 片段插入的效率进行了全面回顾。这些先进的系统通过减少时间和劳动力加速了微生物工程的发展，并增强了对分子遗传学的理解。此外，还总结了基于 CRISPR/Cas9 的系统在 中生产高附加值化学品和提高应激耐受性方面的研究进展，这对基于 的遗传和合成生物学研究应该具有重要的参考价值。
