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气调贮藏驱动中国稻花香蛋白质组变化。

Controlled atmosphere storage drive proteomic change in Chinese Daohuaxiang.

作者信息

Jie Yu, Shi Tianyu, Zhang Zhongjie

机构信息

National Engineering Research Center for Grain Storage and Transportation, Academy of National Food and Strategic Reserves Administration, PR China.

出版信息

Food Chem X. 2024 Jan 19;21:101005. doi: 10.1016/j.fochx.2023.101005. eCollection 2024 Mar 30.

Abstract

Although the use of a controlled atmosphere is one of the most successful storage techniques, the mechanism thereof in rice storage remains unclear. We stored aromatic rice cultivar Daohuaxiang in a package filled with 98 % N and 35 % CO for 3 months. We investigated 2-acetyl-1-pyrroline loss, enzyme activities, and proteomics changes of rice during storage. The results showed that the content of 2-acetyl-1-pyrroline was reduced by 37.40 %, 25.65 %, and 43.89 % during storage using 98 % N, 35 % CO controlled atmosphere storage, and conventional storage. Controlled atmosphere storage slowed down the increase of malondialdehyde content in Daohuaxiang. The results showed that 26S proteasome regulatory particle triple-A ATPase subunit 6, superoxide dismutase, glutathione transferase, and other key proteins were upregulated during 35 % CO regulation. This study provided a meaningful basis for exploring the regulation strategy of aromatic rice quality and strengthening the quality control of aromatic rice industry.

摘要

尽管采用气调贮藏是最成功的贮藏技术之一,但其在稻谷贮藏中的作用机制尚不清楚。我们将香稻品种稻花香贮藏于充满98%氮气和35%二氧化碳的包装袋中3个月。我们研究了贮藏期间稻谷中2-乙酰基-1-吡咯啉的损失、酶活性和蛋白质组学变化。结果表明,在使用98%氮气、35%二氧化碳气调贮藏和常规贮藏期间,2-乙酰基-1-吡咯啉的含量分别降低了37.40%、25.65%和43.89%。气调贮藏减缓了稻花香中丙二醛含量的增加。结果表明,在35%二氧化碳调节期间,26S蛋白酶体调节颗粒三磷酸腺苷酶亚基6、超氧化物歧化酶、谷胱甘肽转移酶等关键蛋白上调。本研究为探索香稻品质调控策略和加强香稻产业质量控制提供了有意义的依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ed/10847794/df8439b3ab23/gr1.jpg

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