Vercesi A E
An Acad Bras Cienc. 1985 Sep;57(3):369-75.
Experiments in this paper demonstrate that mitochondrial damage associated to NAD(P)+-induced Ca2+ efflux, is the consequence of inappropriate reaction conditions. The major findings are (i) Added oxaloacetate and acetoacetate readily oxidize NAD(P)H in intact rat liver mitochondria without causing swelling or membrane damage. (ii) Ca2+ efflux can be induced by the oxidized state of mitochondrial NADP in the presence of ATP, ADP, Pi and Mg2+, without mitochondrial swelling. (iii) Ca2+ efflux induced by NADP+ in the presence of ruthenium red causes no significant impairment of respiratory control, a sensitive measure of membrane potential.
本文中的实验表明，与NAD(P)+诱导的Ca2+外流相关的线粒体损伤是反应条件不当的结果。主要发现如下：(i) 添加的草酰乙酸和乙酰乙酸能在完整的大鼠肝线粒体中轻易氧化NAD(P)H，而不会引起肿胀或膜损伤。(ii) 在ATP、ADP、Pi和Mg2+存在的情况下，线粒体NADP的氧化态可诱导Ca2+外流，而不会导致线粒体肿胀。(iii) 在钌红存在的情况下，NADP+诱导的Ca2+外流不会对呼吸控制产生显著损害，呼吸控制是膜电位的一项敏感指标。
