Vercesi A E
Arch Biochem Biophys. 1987 Jan;252(1):171-8. doi: 10.1016/0003-9861(87)90021-x.
The pyridine nucleotide specificity, the participation of delta psi, and the energy-linked transhydrogenase in the process of Ca2+ efflux stimulated by the oxidized state of NAD(P) were examined in rat liver mitochondria energized by ascorbate + TMPD. The following observations were made: The Ca2+ efflux rate is independent of the redox state of mitochondrial NAD, but is at a minimum when mitochondrial NADP is in the reduced state and accelerated several-fold when it is in the oxidized state. When the redox state of NADP is shifted to a more oxidized state, the steady-state level of Ca2+ in the medium increased and delta psi decreased in proportion to the mitochondrial NADP+ level. The activity of the energy-linked NAD(P) transhydrogenase seems to be a key element in determining the redox state of NADP and thus of Ca2+ retention and efflux from mitochondria.
在由抗坏血酸 + TMPD 供能的大鼠肝线粒体中，研究了吡啶核苷酸特异性、Δψ 的参与以及能量偶联转氢酶在 NAD(P) 氧化态刺激的 Ca2+ 外流过程中的作用。得到以下观察结果：Ca2+ 外流速率与线粒体 NAD 的氧化还原状态无关，但在线粒体 NADP 处于还原态时降至最低，而当其处于氧化态时则加速数倍。当 NADP 的氧化还原状态转变为更氧化的状态时，培养基中 Ca2+ 的稳态水平升高，并且 Δψ 与线粒体 NADP+ 水平成比例下降。能量偶联的 NAD(P) 转氢酶的活性似乎是决定 NADP 氧化还原状态从而决定 Ca2+ 在线粒体中的保留和外流的关键因素。
