Zajicek G, Michaeli Y, Regev J
Cell Tissue Kinet. 1979 May;12(3):229-37. doi: 10.1111/j.1365-2184.1979.tb00145.x.
A method is described, which is applicable to cell renewal systems with an anatomical structure in which all cell locations may be uniquely mapped. Its use is demonstrated on the rat incisor inner enamel epithelium, which forms a one cell thick column in the sagittally sectioned tooth. Cells born in the apical part of the column migrate toward the distal end of the tooth, where they mature. As the cells migrate along the column, they traverse the various cell cycle phases. The present study has been designed to estimate the probability of a cell being in a given phase; all cells touching the basement membrane were numbered, and the number of cells separating any two cells was taken as a measure of distance. Since generally all cells move in one direction (lateral cell migration may occur), it is possible to solve the problem with the aid of functions describing the renewal counting stochastic process in which cell distance serves as an independent variable. The method predicts labelled cell and mitotic rates which agree with those estimated in the usual way. It was then utilized to estimate the fraction of cells in G2.
本文描述了一种方法，该方法适用于具有解剖结构的细胞更新系统，其中所有细胞位置都可以唯一映射。该方法在大鼠切牙内釉上皮上得到了验证，在矢状切片的牙齿中，内釉上皮形成一层细胞厚的柱状结构。在柱状结构顶端产生的细胞向牙齿远端迁移，并在那里成熟。当细胞沿着柱状结构迁移时，它们经历不同的细胞周期阶段。本研究旨在估计细胞处于特定阶段的概率；对所有接触基底膜的细胞进行编号，并将任意两个细胞之间的细胞数量作为距离的度量。由于通常所有细胞都朝一个方向移动（可能会发生侧向细胞迁移），因此借助描述更新计数随机过程的函数来解决该问题成为可能，其中细胞距离作为自变量。该方法预测的标记细胞率和有丝分裂率与以常规方式估计的结果一致。然后利用该方法估计处于G2期的细胞比例。
