Division of Basic Science and Translational Research, Department of Obstetrics and Gynaecology, The University of Texas Medical Branch at Galveston, Galveston, TX, USA.
Department of Biochemistry and Molecular Biology, College of Medicine, University of the Philippines Manila, Manila, Philippines.
Biol Reprod. 2024 May 9;110(5):950-970. doi: 10.1093/biolre/ioae026.
Research on the biology of fetal-maternal barriers has been limited by access to physiologically relevant cells, including trophoblast cells. In this study, we describe the development of a human term placenta-derived cytotrophoblast immortalized cell line (hPTCCTB) derived from the basal plate. Human-term placenta-derived cytotrophoblast immortalized cell line cells are comparable to their primary cells of origin in terms of morphology, marker expression, and functional responses. We demonstrate that these can transform into syncytiotrophoblast and extravillous trophoblasts. We also compared the hPTCCTB cells to immortalized chorionic trophoblasts (hFM-CTC), trophoblasts of the chorionic plate, and BeWo cells, choriocarcinoma cell lines of conventional use. Human-term placenta-derived cytotrophoblast immortalized cell line and hFM-CTCs displayed more similarity to each other than to BeWos, but these differ in syncytialization ability. Overall, this study (1) demonstrates that the immortalized hPTCCTB generated are cells of higher physiological relevance and (2) provides a look into the distinction between the spatially distinct placental and fetal barrier trophoblasts cells, hPTCCTB and hFM-CTC, respectively.
胎儿-母体屏障的生物学研究受到获得生理相关细胞（包括滋养细胞）的限制。在这项研究中，我们描述了一种源自基板的人足月胎盘来源的滋养细胞永生化细胞系（hPTCCTB）的开发。人足月胎盘来源的滋养细胞永生化细胞系在形态、标志物表达和功能反应方面与它们的原始细胞相似。我们证明这些细胞可以转化为合体滋养层细胞和绒毛外滋养层细胞。我们还将 hPTCCTB 细胞与永生化绒毛膜滋养层细胞（hFM-CTC）、绒毛膜板滋养层细胞和 BeWo 细胞进行了比较，BeWo 细胞是常规使用的绒毛膜癌细胞系。人足月胎盘来源的滋养细胞永生化细胞系和 hFM-CTCs 彼此之间的相似性高于 BeWo 细胞，但它们在合胞体形成能力上存在差异。总的来说，这项研究（1）证明了所产生的永生化 hPTCCTB 是更具生理相关性的细胞，（2）提供了对空间上不同的胎盘和胎儿屏障滋养层细胞（hPTCCTB 和 hFM-CTC）之间区别的观察。
