Mohammadzadeh Jahangir, Raoufy Mohammad Reza, Nazari Milad, Sajedi Reza H
Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
Department of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
J Photochem Photobiol B. 2024 Mar;252:112852. doi: 10.1016/j.jphotobiol.2024.112852. Epub 2024 Jan 28.
Infertility is such an important issue in society today. In some cases of male infertility, the main cause is oxidative stress and the presence of reactive oxygen species in the environment or in sperm cells. All current techniques that measure oxidative stress, including the nitroblue tetrazolium Test, DNA Fragmentation Index, Malondialdehyde, and Endz Test are qualitative and semi-quantitative. These methods do not have good sensitivity and specificity. Semen samples from 50 infertile patients and 10 normal individuals were collected. The samples were examined for laboratory routine tests according to the WHO 2010 protocol. Oxidative stress tests, including DFI, NBT, and MDA, were performed for these two groups. Bioluminescence inhibition assay was performed for detection of O in semen samples by aequorin. The normal individuals showed significantly better semen parameters than the patient's group. Significantly lower O levels were seen in the patient's group compared to normal individuals. The cut-off value of O levels in normal individuals was determined to be 8 × 10 RLU/s with a sensitivity of 100% and a specificity of 100%. Infertile patients, despite having reduced quality of semen parameters, have high O levels, and this causes the intensity of bioluminescence to be quenched in these people.
不孕不育是当今社会的一个重要问题。在某些男性不育病例中，主要原因是氧化应激以及环境或精子细胞中活性氧的存在。目前所有测量氧化应激的技术，包括硝基蓝四唑试验、DNA碎片化指数、丙二醛和Endz试验，都是定性和半定量的。这些方法的灵敏度和特异性都不高。收集了50例不孕患者和10例正常个体的精液样本。根据世界卫生组织2010年方案对样本进行实验室常规检测。对这两组进行了氧化应激试验，包括DFI、NBT和MDA。通过水母发光蛋白对精液样本进行生物发光抑制试验以检测O。正常个体的精液参数明显优于患者组。与正常个体相比，患者组的O水平明显较低。正常个体的O水平临界值确定为8×10 RLU/s，灵敏度为100%，特异性为100%。不孕患者尽管精液参数质量下降，但O水平较高，这导致这些人的生物发光强度被淬灭。
