Barres D R
Ann Pathol. 1985;5(4-5):259-64.
Computer modelling is used to simulate nuclear segments obtained by random sectioning through tissue. A few more computations lead to DNA measurement simulation. Simulation results in negatively skewed DNA frequency distributions. Both the right skewness and the coefficient of variation of measurements are increasing with the ploidy level because nuclear DNA content is assumed to be related to nuclear size in the chosen model. Observed mean values are biased underestimates of expected values but are strongly correlated to the degree of ploidy. Finally, the bias introduced by measuring nuclear segments instead of whole nuclei increases the variance of measurements but contributes to less than half the experimentally observed variance. Our conclusion is that microspectrophotometry on tissue sections is a valuable method for DNA content evaluation of small clusters of pathological cells as one may find in endoscopic biopsies.
计算机建模用于模拟通过对组织进行随机切片获得的细胞核片段。再进行一些计算就可实现DNA测量模拟。模拟结果得出呈负偏态的DNA频率分布。由于在所选模型中假定核DNA含量与核大小相关，因此测量值的右偏度和变异系数均随倍性水平的增加而增加。观察到的平均值是对期望值的有偏低估，但与倍性程度密切相关。最后，通过测量细胞核片段而非完整细胞核所引入的偏差增加了测量的方差，但在实验观察到的方差中所占比例不到一半。我们的结论是，对组织切片进行显微分光光度测定是评估病理细胞小簇DNA含量的一种有价值的方法，就像在内镜活检中可能发现的那样。
