Iiams Samantha E, Wan Guijun, Zhang Jiwei, Lugena Aldrin B, Zhang Ying, Hayden Ashley N, Merlin Christine
Department of Biology and Center for Biological Clocks Research, Texas A&M University, College Station, TX 77843, USA.
Genetics Interdisciplinary Program, Texas A&M University, College Station, TX 77843, USA.
iScience. 2024 Jan 20;27(2):108980. doi: 10.1016/j.isci.2024.108980. eCollection 2024 Feb 16.
Light is one of the strongest cues for entrainment of circadian clocks. While some insect species rely only on visual input, others like use both the visual system and the deep-brain blue-light photoreceptor cryptochrome for entraining circadian rhythms. Here, we used the monarch butterfly (), which possesses a light-sensitive (), to test the conservation of mechanisms of clock entrainment. We showed that loss of functional reduced the amplitude and altered the phase of adult eclosion rhythms, and disrupted brain molecular circadian rhythms. Robust rhythms could be restored by entrainment to temperature cycles, indicating a likely functional core circadian clock in mutants. We also showed that rhythmic flight activity was less robust in mutants, and that visual impairment in mutants impacted flight suppression at night. Our data suggest that dpCRY1 is a major photoreceptor for light-entrainment of the monarch circadian clock.
光 是昼夜节律时钟同步的最强线索之一。虽然一些昆虫物种仅依赖视觉输入，但其他物种（如 ）则同时利用视觉系统和大脑深层蓝光光感受器隐花色素来同步昼夜节律。在这里，我们使用了拥有光敏感 （ ）的黑脉金斑蝶（ ）来测试时钟同步机制的保守性。我们发现功能性 的缺失降低了成虫羽化节律的幅度并改变了其相位，还扰乱了大脑分子昼夜节律。通过温度循环同步可以恢复稳健的节律，这表明 突变体中可能存在功能性核心昼夜节律时钟。我们还表明， 突变体中有节奏的飞行活动不太稳健，并且 突变体中的视觉损伤会影响夜间飞行抑制。我们的数据表明，dpCRY1 是黑脉金斑蝶昼夜节律时钟光同步的主要光感受器。
