Pak J Biol Sci. 2023 Nov;26(12):628-650. doi: 10.3923/pjbs.2023.628.650.
<b>Background and Objective:</b> Cerebellar fluorosis is a health issue associated with excessive exposure to fluoride (F) either in direct or indirect ways as pesticides, drinking water and caries preventing prescriptions. It is characterized by elevation in oxidative stress, inflammation, demyelination and Purkinje cell loss. <i>Moringa oleifera</i> (M), is a widely cultivated plant used as a health-booster agent in modulating various disorders because of its high content of vitamins and minerals. The beneficial effect of moringa against fluoride-induced cerebellar toxicity in pregnant rats was investigated in this study. <b>Materials and Methods:</b> Twenty pregnant rats were administered daily 300 mg kg<sup></sup><sup>1</sup> <i>M. oleifera</i> aqueous extract incorporated with 10 mg kg<sup></sup><sup>1</sup> of F intoxication from the 1st day of gestation until the 20th day. Following the termination of the trial, sera were collected and cerebellar tissue was removed for further examinations, along with the assessment of maternity. <b>Results:</b> The <i>M. oleifera</i> significantly normalized serum FSH, LH, progesterone, dopamine and serotonin levels of F-intoxicated mothers. Additionally, <i>M. oleifera</i> markedly prevented the lipid peroxidation and DNA fragmentation indicated by the tail length and moment in comet assay (-34.4 and -75.3%, respectively, when compared to the fluoride intoxicated group), while sustaining the levels of SOD and CAT revealing its antioxidant activity. The <i>M. oleifera</i> regressed the cerebellar α-amylase (-25.4%) and acetylcholinesterase activity (-40.6%), also attenuated GFAP (-73.4%, p<0.0001), synaptophysin level (216.6%, p<0.0001) and IL-6 expression (-91.2%) comparing to fluoride only treated mothers. <b>Conclusion:</b> Histological and ultrastructural examinations confirmed the recuperating effects of <i>M. oleifera</i> on mothers' cerebellar tissue intoxicated with fluoride indicated by intact folia and restored Purkinje cells number and architecture. The maternal study emphasized the anti-abortifacient activity of moringa against fluoride induced-fetotoxicity.
氟化物（F）无论是直接还是间接的方式，例如杀虫剂、饮用水和龋齿预防处方，过度暴露都会导致小脑氟中毒，这是一个健康问题。它的特征是氧化应激、炎症、脱髓鞘和浦肯野细胞丢失增加。辣木（Moringa oleifera）（M）是一种广泛种植的植物，由于其富含维生素和矿物质，被用作调节各种疾病的健康增强剂。本研究旨在探讨辣木对氟诱导的怀孕大鼠小脑毒性的有益作用。材料与方法：20 只怀孕大鼠从妊娠第 1 天到第 20 天每天给予 300mg/kg<sup></sup><sup>1</sup>辣木的水提取物，同时给予 10mg/kg<sup></sup><sup>1</sup>的氟化物中毒。试验结束后，收集血清，取出小脑组织进行进一步检查，并评估孕鼠情况。结果：辣木显著调节了氟中毒母亲的血清 FSH、LH、孕酮、多巴胺和血清素水平。此外，辣木显著防止了脂质过氧化和 DNA 片段化，彗星试验中的尾长和力矩分别降低了-34.4%和-75.3%（与氟化物中毒组相比），同时维持了 SOD 和 CAT 的水平，显示出其抗氧化活性。辣木降低了小脑α-淀粉酶（-25.4%）和乙酰胆碱酯酶活性（-40.6%），还减弱了 GFAP（-73.4%，p<0.0001）、突触小体蛋白水平（216.6%，p<0.0001）和 IL-6 表达（-91.2%），与单独用氟化物处理的母亲相比。结论：组织学和超微结构检查证实，辣木对氟化物中毒的母亲小脑组织有恢复作用，表现为完整的小叶和恢复的浦肯野细胞数量和结构。母体研究强调了辣木对氟化物诱导的胎儿毒性的抗流产活性。
