National Research University "Higher School of Economics", Moscow, Russia.
National Medical Research Radiological Center, Ministry of Health of Russia, Moscow, Russia.
Dokl Biochem Biophys. 2023 Dec;513(Suppl 1):S96-S99. doi: 10.1134/S1607672923700692. Epub 2024 Feb 9.
It was proven that tumor organoids effectively mirror the phenotypic and genetic traits of the original biomaterial. It was reported that outcomes from drug testing in organoid cultures can accurately represent the clinical response observed in patients. In this study, an organoid culture was derived from biopsy material of prostate cancer (PC). Subsequently, clinical practice drugs, docetaxel and enzalutamide, were tested on this organoid culture. Various techniques for evaluating the efficacy of drugs in vitro were compared. The half-maximal inhibitory concentration of docetaxel was found to be markedly lower compared to that of enzalutamide. However, when tested at clinically relevant concentrations and incubation times, enzalutamide was more effective than docetaxel. Therefore, it is crucial to optimize the testing conditions for drugs on in vitro cultures for their subsequent application in clinical practice.
已经证明肿瘤类器官有效地反映了原始生物材料的表型和遗传特征。有报道称，在类器官培养物中进行的药物测试结果可以准确反映在患者中观察到的临床反应。在这项研究中，从前列腺癌 (PC) 的活检材料中获得了类器官培养物。随后，在该类器官培养物上测试了临床实践药物多西他赛和恩扎鲁胺。比较了评估体外药物疗效的各种技术。与恩扎鲁胺相比，多西他赛的半最大抑制浓度明显更低。然而，当以临床相关浓度和孵育时间测试时，恩扎鲁胺比多西他赛更有效。因此，对于后续在临床实践中的应用，为药物在体外培养物上的测试条件进行优化至关重要。
