Tian Qiang, Wang Guangzheng, Dou Jianhua, Niu Yu, Li Ruirui, An Wangwang, Tang Zhongqi, Yu Jihua
College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China.
State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China.
Plants (Basel). 2024 Jan 27;13(3):383. doi: 10.3390/plants13030383.
Melatonin plays a vital role in plant growth and development. In this study, we treated hydroponically grown tomato roots with various concentrations of exogenous melatonin (0, 10, 30, and 50 μmol·L). We utilized root scanning and microscopy to examine alterations in root morphology and cell differentiation and elucidated the mechanism by which melatonin regulates these changes through the interplay with endogenous hormones and relevant genes. The results showed that for melatonin at concentrations ranging between 10 and 30 μmol·L, the development of lateral roots were significantly stimulated, the root hair growth was enhanced, and biomass accumulation and root activity were increased. Furthermore, we elucidated that melatonin acts as a mediator for the expression of genes, such as , , , , and , which are involved in the regulation of root morphology changes. Additionally, we observed that melatonin influences the levels of endogenous hormones, including ZT, GA3, IAA, ABA, and BR, which subsequently impact the root morphology development of tomato roots. In summary, this study shows that tomato root morphology can be promoted by the optimal concentration of exogenous melatonin (10-30 μmol·L).
褪黑素在植物生长发育中起着至关重要的作用。在本研究中，我们用不同浓度的外源褪黑素（0、10、30和50 μmol·L）处理水培生长的番茄根。我们利用根系扫描和显微镜检查根系形态和细胞分化的变化，并阐明了褪黑素通过与内源激素和相关基因相互作用调节这些变化的机制。结果表明，对于浓度在10至30 μmol·L之间的褪黑素，侧根的发育受到显著刺激，根毛生长增强，生物量积累和根系活性增加。此外，我们阐明了褪黑素作为参与调节根系形态变化的基因（如 、 、 、 和 ）表达的介质。此外，我们观察到褪黑素影响包括ZT、GA3、IAA、ABA和BR在内的内源激素水平，进而影响番茄根的根系形态发育。总之，本研究表明，外源褪黑素的最佳浓度（10 - 30 μmol·L）可以促进番茄根系形态。
