Mao Wei, Bao Changyan, Cheng Qian, Liang Ning, Wang Lianchun, Yang Hanqi
Faculty of Foreign Languages, Southwest Forestry University, Kunming 650233, China.
Institute of Highland Forest Science, Chinese Academy of Forestry, Kunming 650233, China.
Plants (Basel). 2024 Jan 30;13(3):410. doi: 10.3390/plants13030410.
To explore the regulation mechanism of endogenous phytohormones on rhizome bud germination in , the contents of IAA, ABA, GA, and CTK in seven above- and under-ground bamboo structure components were determined using enzyme-linked immunosorbent assays (ELISA). The results showed that a higher content of IAA, GA, and CTK all year was found in above-ground components and dormant rhizome buds. Meanwhile, a higher ABA content in young shoots and a lower ABA content in the culm base and dormant rhizome buds were detected during the peak period of shooting. The amounts of emerging shoots and the grown bamboo culms were positively correlated with the content of IAA and the ratio of IAA/ABA and (IAA + CTK + GA)/ABA, while they were negatively correlated with the ratio of CTK/IAA in dormant rhizome buds. The all-year high contents of IAA (19-31 ng/g) and ABA (114-144 ng/g) in rhizome buds, as well as interactions among four hormones, may be the key physiological mechanisms to maintain rhizome bud germination throughout the year in . As is a special bamboo species of multi-season shoot sprouting, the above results may supplement scientific data for a comprehensive understanding of physiological mechanisms within the bamboo subfamily.
为探究内源植物激素对[具体竹种]根茎芽萌发的调控机制，采用酶联免疫吸附测定法（ELISA）测定了7种地上和地下竹结构组分中吲哚乙酸（IAA）、脱落酸（ABA）、赤霉素（GA）和细胞分裂素（CTK）的含量。结果表明，地上部分和休眠根茎芽中IAA、GA和CTK全年含量较高。同时，在出笋高峰期，幼笋中ABA含量较高，而竹秆基部和休眠根茎芽中ABA含量较低。出笋数量和生长的竹秆与IAA含量以及IAA/ABA和（IAA + CTK + GA）/ABA的比值呈正相关，而与休眠根茎芽中CTK/IAA的比值呈负相关。根茎芽中全年较高的IAA（19 - 31 ng/g）和ABA（114 - 144 ng/g）含量以及四种激素之间的相互作用，可能是[具体竹种]全年维持根茎芽萌发的关键生理机制。由于[具体竹种]是一种多季出笋的特殊竹种，上述结果可能为全面了解竹亚科内的生理机制补充科学数据。
