水果多糖：提取、纯化、结构表征及抗氧化活性。

Polysaccharides from Fruit: Extraction, Purification, Structural Characterization, and Antioxidant Activities.

机构信息

College of Food Science and Technology, Hebei Normal University of Science & Technology, Qinhuangdao 066600, China.

College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.

出版信息

Molecules. 2023 Jan 28;28(3):1266. doi: 10.3390/molecules28031266.

Polysaccharides are important bioactive components of fruit (NRF). In this study, the ultrasonic-assisted extraction (UAE) conditions of polysaccharides from fruit (NRFPs) were optimized by response surface methodology (RSM). The structural characteristics and antioxidant activity were investigated. The maximum NRFPs yield of 3.35% was obtained under the following optimal conditions: temperature of 59.5 °C, time of 30.5 min, liquid-to-solid ratio of 19.5 mL/g. Three polysaccharide fractions, NRFP-1 (20.01 kDa), NRFP-2 (28.96 kDa), and NRFP-3 (67.45 kDa), were isolated. Glucose, galactose, and arabinose in different percentages were identified as the primary monosaccharide units. The Fourier transform infrared spectrometer (FT-IR) and nuclear magnetic resonance (NMR) analysis indicated the presence of α- and β-glycosidic bonds in NRFPs. The NRFP-3 exhibited the highest scavenging activities against DPPH, ABTS, -OH free radicals, and Fe-reducing activity.

多糖是水果（NRF）中重要的生物活性成分。在本研究中，采用响应面法（RSM）对水果（NRFPs）多糖的超声辅助提取（UAE）条件进行了优化。研究了其结构特征和抗氧化活性。在以下最佳条件下，可获得 3.35%的最大 NRFPs 产率：温度为 59.5°C，时间为 30.5 分钟，液固比为 19.5mL/g。分离得到三种多糖级分，NRFP-1（20.01 kDa）、NRFP-2（28.96 kDa）和 NRFP-3（67.45 kDa）。鉴定出不同比例的葡萄糖、半乳糖和阿拉伯糖为主要单糖单元。傅里叶变换红外光谱（FT-IR）和核磁共振（NMR）分析表明，NRFPs 中存在α-和β-糖苷键。NRFP-3 对 DPPH、ABTS、-OH 自由基和 Fe 还原活性的清除活性最高。