Rathod Gunvantsinh, Amamcharla Jayendra
Department of Animal Sciences and Industry, Food Science Institute, Kansas State University, Manhattan, KS 66506, USA.
Idaho Milk Products, Jerome, ID 83338, USA.
Foods. 2024 Feb 1;13(3):466. doi: 10.3390/foods13030466.
Milk whey proteins, which are derived from skim milk through membrane filtration, exhibit valuable functional properties when transformed into a fibrillar form. This conversion enhances their suitability for various applications, including thickening, gelling, emulsification, and foaming. However, reported fibrillation methods have longer heating times, which may not be economical for the dairy industry. To address these challenges, the current study was undertaken with the objective of reducing the time required for fibril formation. In this study, 2% milk whey protein isolate (mWPI) solution at pH 2 was heated with static and stirring heating conditions at 80 °C for 20 h to convert milk whey proteins into fibrils. Fibrils were observed using the thioflavin T value, transmission electron microscopy, Tricine SDS-PAGE, rheology, and protein oxidation. Results suggest that stirring heating conditions with 14 h heating time produced fibrils with good morphology compared to static heating, showing a 6 h reduction compared to an earlier reported 80 °C for 20 h heating time. Also, stirring heating produced a uniform and homogeneous fibril solution compared to the static heating method. Gentle stirring during heating can also help to scale up fibril production in an industrial setup. The fibrillation method with processing intervention will help to produce fibrils with enhanced functionality at the pilot and industrial scales.
乳清蛋白是通过膜过滤从脱脂牛奶中提取的，当转化为纤维状形式时，具有宝贵的功能特性。这种转化提高了它们在各种应用中的适用性，包括增稠、胶凝、乳化和发泡。然而，报道的纤维化方法加热时间较长，这对乳制品行业来说可能不经济。为应对这些挑战，开展了本研究，目的是减少形成纤维所需的时间。在本研究中，将pH值为2的2%乳清蛋白分离物（mWPI）溶液在80°C下分别在静态和搅拌加热条件下加热20小时，以使乳清蛋白转化为纤维。使用硫黄素T值、透射电子显微镜、Tricine SDS-PAGE、流变学和蛋白质氧化来观察纤维。结果表明，与静态加热相比，加热14小时的搅拌加热条件产生的纤维形态良好，与先前报道的80°C加热20小时相比减少了6小时。此外，与静态加热方法相比，搅拌加热产生了均匀且均质的纤维溶液。加热过程中的温和搅拌也有助于在工业装置中扩大纤维生产规模。采用加工干预的纤维化方法将有助于在中试和工业规模上生产具有增强功能的纤维。
